Using FRET-based reporters to visualize subcellular dynamics of protein kinase A activity

Charlene Depry, Jin Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

The ubiquitous Protein Kinase A (PKA) signaling pathway is responsible for the regulation of numerous processes including gene expression, metabolism, cell growth, and cell proliferation. This method details how to monitor real-time PKA activity dynamics in mammalian cells using fluorescence resonance energy transfer (FRET)-based reporters.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages285-294
Number of pages10
Volume756
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume756
ISSN (Print)10643745

Keywords

  • Fluorescence resonance energy transfer
  • Kinase activity reporter
  • Live-cell imaging
  • Protein kinase A

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Using FRET-based reporters to visualize subcellular dynamics of protein kinase A activity'. Together they form a unique fingerprint.

Cite this