Using delayed decoupling to attenuate residual signals in editing filters

Kenneth A. Marincin, Indrani Pal, Dominique P. Frueh

Research output: Contribution to journalArticlepeer-review


Isotope filtering methods are instrumental in biomolecular nuclear magnetic resonance (NMR) studies as they isolate signals of chemical moieties of interest within complex molecular assemblies. However, isotope filters suppress undesired signals of isotopically enriched molecules through scalar couplings, and variations in scalar couplings lead to imperfect suppressions, as occurs for aliphatic and aromatic moieties in proteins. Here, we show that signals that have escaped traditional filters can be attenuated with mitigated sensitivity losses for the desired signals of unlabeled moieties. The method uses a shared evolution between the detection and preceding preparation period to establish non-observable antiphase coherences and eliminates them through composite pulse decoupling. We demonstrate the method by isolating signals of an unlabeled post-translational modification tethered to an isotopically enriched protein.

Original languageEnglish (US)
Pages (from-to)475-487
Number of pages13
JournalMagnetic Resonance
Issue number1
StatePublished - 2021

ASJC Scopus subject areas

  • Analytical Chemistry
  • Condensed Matter Physics
  • Atomic and Molecular Physics, and Optics


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