Abstract
Molecular switches have been instrumental in the development of powerful and versatile genetic tools for directly probing biochemical processes, such as intracellular signaling, within their native contexts. A molecular switch can be broadly defined as a molecular system capable of existing in either of two states (e.g., conformations), which can be converted from one state to the other by a specific input stimulus. This protocol outlines a method for using a kinase-inducible bimolecular switch, along with live-cell fluorescence microscopy, to directly control and monitor the activity of a specific enzyme in living cells.
Original language | English (US) |
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Pages (from-to) | 227-237 |
Number of pages | 11 |
Journal | Current protocols in chemical biology |
Volume | 5 |
Issue number | 3 |
DOIs | |
State | Published - 2013 |
Keywords
- biochemical perturbation
- biosensor
- fluorescence
- live-cell imaging
ASJC Scopus subject areas
- General Medicine