Using a kinase-inducible bimolecular switch to control enzyme activity in living cells

Sohum Mehta, Jin Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

Molecular switches have been instrumental in the development of powerful and versatile genetic tools for directly probing biochemical processes, such as intracellular signaling, within their native contexts. A molecular switch can be broadly defined as a molecular system capable of existing in either of two states (e.g., conformations), which can be converted from one state to the other by a specific input stimulus. This protocol outlines a method for using a kinase-inducible bimolecular switch, along with live-cell fluorescence microscopy, to directly control and monitor the activity of a specific enzyme in living cells.

Original languageEnglish (US)
Pages (from-to)227-237
Number of pages11
JournalCurrent protocols in chemical biology
Volume5
Issue number3
DOIs
StatePublished - 2013

Keywords

  • biochemical perturbation
  • biosensor
  • fluorescence
  • live-cell imaging

ASJC Scopus subject areas

  • Medicine(all)

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