Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells

Sohum Mehta, Jin Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Calcineurin is an evolutionarily conserved, ubiquitously expressed protein phosphatase that serves as a major effector of Ca2+ signals, regulating diverse biological processes such as gene expression, tissue differentiation, immune responses, and neural plasticity. The following method describes how to monitor real-time calcineurin activity in cultured mammalian cells using a fluorescence resonance energy transfer (FRET)-based activity reporter.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages139-149
Number of pages11
Volume1071
ISBN (Print)9781627036214
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1071
ISSN (Print)10643745

Keywords

  • Biosensor
  • Calcineurin
  • Calmodulin
  • FRET
  • Live-cell imaging
  • NFAT
  • Phosphatase activity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Mehta, S., & Zhang, J. (2014). Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells. In Methods in Molecular Biology (Vol. 1071, pp. 139-149). (Methods in Molecular Biology; Vol. 1071). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-622-1_11