Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells

Sohum Mehta, Jin Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Calcineurin is an evolutionarily conserved, ubiquitously expressed protein phosphatase that serves as a major effector of Ca2+ signals, regulating diverse biological processes such as gene expression, tissue differentiation, immune responses, and neural plasticity. The following method describes how to monitor real-time calcineurin activity in cultured mammalian cells using a fluorescence resonance energy transfer (FRET)-based activity reporter.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages139-149
Number of pages11
Volume1071
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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