TY - CHAP
T1 - Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells
AU - Mehta, Sohum
AU - Zhang, Jin
PY - 2014
Y1 - 2014
N2 - Calcineurin is an evolutionarily conserved, ubiquitously expressed protein phosphatase that serves as a major effector of Ca2+ signals, regulating diverse biological processes such as gene expression, tissue differentiation, immune responses, and neural plasticity. The following method describes how to monitor real-time calcineurin activity in cultured mammalian cells using a fluorescence resonance energy transfer (FRET)-based activity reporter.
AB - Calcineurin is an evolutionarily conserved, ubiquitously expressed protein phosphatase that serves as a major effector of Ca2+ signals, regulating diverse biological processes such as gene expression, tissue differentiation, immune responses, and neural plasticity. The following method describes how to monitor real-time calcineurin activity in cultured mammalian cells using a fluorescence resonance energy transfer (FRET)-based activity reporter.
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U2 - 10.1007/978-1-62703-622-1-11
DO - 10.1007/978-1-62703-622-1-11
M3 - Chapter
C2 - 24052386
VL - 1071
SP - 139
EP - 149
BT - Methods in Molecular Biology
ER -