Calcineurin is an evolutionarily conserved, ubiquitously expressed protein phosphatase that serves as a major effector of Ca2+ signals, regulating diverse biological processes such as gene expression, tissue differentiation, immune responses, and neural plasticity. The following method describes how to monitor real-time calcineurin activity in cultured mammalian cells using a fluorescence resonance energy transfer (FRET)-based activity reporter.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Number of pages||11|
|State||Published - 2014|
ASJC Scopus subject areas
- Molecular Biology