TY - JOUR
T1 - Usefulness of soluble and surface-bound P-selectin in detecting heightened platelet activity in patients with congestive heart failure
AU - O'Connor, Christopher M.
AU - Gurbel, Paul A.
AU - Serebruany, Victor L.
N1 - Funding Information:
This study was supported in part by Centocor Diagnostics of Pennsylvania, Malvern, Pennsylvania. Manuscript received October 16, 1998; revised manuscript received and accepted January 1, 1999.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999/5/1
Y1 - 1999/5/1
N2 - P-selectin is an important marker of platelet activation and may be up- regulated in patients with congestive heart failure (CHF). We sought to prospectively compare simultaneously determined platelet and soluble P- selectin in patients with CHF and in healthy controls. Matched soluble levels by enzyme-linked immmunosorbent assay, and platelet-bound expression by whole blood flow cytometry of P-selectin were measured in 22 patients with CHF and compared with 14 healthy controls. Twelve patients were aspirin free and 10 patients were taking aspirin (81 to 500 mg/day). Patients with CHF had significantly elevated soluble P-selectin (186.6 ± 82.7 ng/ml, p = 0.017) and more than twofold increased expression of platelet-bound P-selectin (9.58 ± 7.16% of positive platelets, p = 0.021) compared with the P-selectin profile (102.6 ± 29.0 ng/ml of plasma, and 4.06 ± 1.21% of positive platelets, respectively) in controls. Aspirin therapy did not affect the P- selectin profile in patients with CHF. Despite antecedent aspirin therapy and interindividual variability of the P-selectin profile, soluble and platelet P-selectin were elevated in most patients with severe CHF, suggesting persistent platelet activation.
AB - P-selectin is an important marker of platelet activation and may be up- regulated in patients with congestive heart failure (CHF). We sought to prospectively compare simultaneously determined platelet and soluble P- selectin in patients with CHF and in healthy controls. Matched soluble levels by enzyme-linked immmunosorbent assay, and platelet-bound expression by whole blood flow cytometry of P-selectin were measured in 22 patients with CHF and compared with 14 healthy controls. Twelve patients were aspirin free and 10 patients were taking aspirin (81 to 500 mg/day). Patients with CHF had significantly elevated soluble P-selectin (186.6 ± 82.7 ng/ml, p = 0.017) and more than twofold increased expression of platelet-bound P-selectin (9.58 ± 7.16% of positive platelets, p = 0.021) compared with the P-selectin profile (102.6 ± 29.0 ng/ml of plasma, and 4.06 ± 1.21% of positive platelets, respectively) in controls. Aspirin therapy did not affect the P- selectin profile in patients with CHF. Despite antecedent aspirin therapy and interindividual variability of the P-selectin profile, soluble and platelet P-selectin were elevated in most patients with severe CHF, suggesting persistent platelet activation.
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U2 - 10.1016/S0002-9149(99)00098-3
DO - 10.1016/S0002-9149(99)00098-3
M3 - Article
C2 - 10235093
AN - SCOPUS:0033135060
SN - 0002-9149
VL - 83
SP - 1345
EP - 1349
JO - American Journal of Cardiology
JF - American Journal of Cardiology
IS - 9
ER -