Use of vascularized abdominal wall pedicle flaps to grow small bowel neomucosa

Keith D. Lillemoe, William R. Berry, John Harmon, Y. H. Tai, Robert H. Weichbrod, Mark A. Cogen

Research output: Contribution to journalArticle

Abstract

Growing new mucosa from remnants of small bowel remaining in patients with short-bowel syndrome might offer a strategy for solving this clinical problem. We have performed a series of experiments investigating the possibility of growing rabbit ileal mucosa on vascularized pedicle flaps of abdominal wall musculature based on the inferior epigastric artery. By patching a defect of distal ileum with a skeletal muscle flap, we were able to demonstrate bowel augmentation by neomucosal ingrowth. Light and scanning electron microscopy confirmed the presence of essentially normal mucosa with well-developed villi atop the skeletal muscle pedicle flap. The mucosa was stripped from the skeletal muscle and compared with stripped mucosa from adjacent ileum in the Ussing chamber in 11 rabbits. The electrophysiologic studies showed no significant difference between normal mucosa and neomucosa in short-circuit current (Isc), potential difference, or tissue conductance. The addition of 10 mM glucose resulted in similar unidirectional glucose flux and increase in Isc in both tissues. Bile salt absorption was also similar in both tissues. We conclude that neomucosa can be grown on flaps of skeletal muscle and is similar to normal mucosa by microscopic and electrophysiologic evaluation.

Original languageEnglish (US)
Pages (from-to)293-300
Number of pages8
JournalSurgery
Volume91
Issue number3
StatePublished - 1982
Externally publishedYes

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Abdominal Wall
Mucous Membrane
Skeletal Muscle
Ileum
Epigastric Arteries
Rabbits
Short Bowel Syndrome
Glucose
Bile Acids and Salts
Electron Scanning Microscopy
Light

ASJC Scopus subject areas

  • Surgery

Cite this

Lillemoe, K. D., Berry, W. R., Harmon, J., Tai, Y. H., Weichbrod, R. H., & Cogen, M. A. (1982). Use of vascularized abdominal wall pedicle flaps to grow small bowel neomucosa. Surgery, 91(3), 293-300.

Use of vascularized abdominal wall pedicle flaps to grow small bowel neomucosa. / Lillemoe, Keith D.; Berry, William R.; Harmon, John; Tai, Y. H.; Weichbrod, Robert H.; Cogen, Mark A.

In: Surgery, Vol. 91, No. 3, 1982, p. 293-300.

Research output: Contribution to journalArticle

Lillemoe, KD, Berry, WR, Harmon, J, Tai, YH, Weichbrod, RH & Cogen, MA 1982, 'Use of vascularized abdominal wall pedicle flaps to grow small bowel neomucosa', Surgery, vol. 91, no. 3, pp. 293-300.
Lillemoe KD, Berry WR, Harmon J, Tai YH, Weichbrod RH, Cogen MA. Use of vascularized abdominal wall pedicle flaps to grow small bowel neomucosa. Surgery. 1982;91(3):293-300.
Lillemoe, Keith D. ; Berry, William R. ; Harmon, John ; Tai, Y. H. ; Weichbrod, Robert H. ; Cogen, Mark A. / Use of vascularized abdominal wall pedicle flaps to grow small bowel neomucosa. In: Surgery. 1982 ; Vol. 91, No. 3. pp. 293-300.
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