Use of the Roche LightCycler Instrument in a Real-Time PCR for Trichomonas vaginalis in Urine Samples from Females and Males

Justin Hardick, Samuel Yang, Shin Lin, Della Duncan, Charlotte A Gaydos

Research output: Contribution to journalArticle

Abstract

Trichomonas vaginalis is the agent of a highly prevalent sexually transmitted infection (STI) that can result in vaginitis, urethritis, and preterm birth. Traditional methods of diagnosis, including wet preparation, can be unreliable. In this study, we describe the adaptation of an existing PCR method for specific detection of T. vaginalis DNA into a rapid real-time PCR assay based on fluorescence resonance energy transfer (FRET) probe chemistry. The FRET-based assay described demonstrated high sensitivity with a detection limit of 1.06 organisms, as well as a high specificity. A total of 253 urine samples collected prospectively from both men and women were tested for T. vaginalis DNA with both the FRET-based assay and a previously validated PCR assay. When the validated PCR assay was used as the "gold standard" and after discrepancies had been resolved, our FRET-based assay demonstrated an analytical sensitivity and specificity of 90.1 and 100%, respectively. Overall results suggest that FRET-based assays can provide rapid, accurate, and high-throughput detection of T. vaginalis and may prove useful in clinical settings and for large-scale screening programs.

Original languageEnglish (US)
Pages (from-to)5619-5622
Number of pages4
JournalJournal of Clinical Microbiology
Volume41
Issue number12
DOIs
StatePublished - Dec 2003

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Trichomonas vaginalis
Fluorescence Resonance Energy Transfer
Real-Time Polymerase Chain Reaction
Urine
Polymerase Chain Reaction
Vaginitis
Urethritis
Premature Birth
Sexually Transmitted Diseases
Limit of Detection
Sensitivity and Specificity

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Use of the Roche LightCycler Instrument in a Real-Time PCR for Trichomonas vaginalis in Urine Samples from Females and Males. / Hardick, Justin; Yang, Samuel; Lin, Shin; Duncan, Della; Gaydos, Charlotte A.

In: Journal of Clinical Microbiology, Vol. 41, No. 12, 12.2003, p. 5619-5622.

Research output: Contribution to journalArticle

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