Use of the quartz crystal microbalance to monitor ligand-induced conformational rearrangements in HIV-1 envelope protein gp120

Hyun Su Lee, Mark Contarino, M. Umashankara, Arne Schön, Ernesto Freire, Amos B. Smith, Irwin M. Chaiken, Lynn S. Penn

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated the potential of a quartz crystal microbalance with dissipation monitoring (QCM-D) to provide a sensitive, label-free method for detecting the conformational rearrangement of glycoprotein gp120 upon binding to different ligands. This glycoprotein is normally found on the envelope of the HIV-1 virus and is involved in viral entry into host cells. It was immobilized on the surface of the sensing element of the QCM-D and was exposed to individual solutions of several different small-molecule inhibitors as well as to a solution of a soluble form of the host cell receptor to which gp120 binds. Instrument responses to ligand-triggered changes were in qualitative agreement with conformational changes as suggested by other biophysical methods.

Original languageEnglish (US)
Pages (from-to)1143-1152
Number of pages10
JournalAnalytical and Bioanalytical Chemistry
Volume396
Issue number3
DOIs
StatePublished - 2010

Keywords

  • Human immunodeficiency virus 1 (HIV-1)
  • Ligand-gp120 complex
  • Quartz crystal microbalance (QCM-D)
  • S-CD4

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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