Use of the polymerase chain reaction to detect hypermethylation in the calcitonin gene. A new, sensitive approach to monitor tumor cells in acute myelogenous leukemia

Takashi Fukuhara, W. Craig Hooper, Stephen B. Baylin, Jane Benson, Janet Pruckler, Anita C. Olson, Bruce L. Evatt, William R. Vogler

Research output: Contribution to journalArticle

Abstract

Based an the recent observations that, in a majority of patients with acute leukemia, the 5′ end of the calcitonin gene was hypermethylated and abnormal DNA fragments were observed following HpaII restriction digestion, we have developed a PCR-based method to sensitively detect this abnormal methylation of the calcitonin gene in AML. Applying the concept of competitive PCR, a semi-quantitative correlation was obtained between the amount of hypermethylation and the amount of leukemic cells present. These results suggest that this method will be useful to monitor the amount of tumor cells in bone marrow from patients with AML.

Original languageEnglish (US)
Pages (from-to)1031-1040
Number of pages10
JournalLeukemia Research
Volume16
Issue number10
DOIs
StatePublished - Oct 1992

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Keywords

  • HL-60
  • HpaII
  • Hypermethylation
  • Southern-blot analysis
  • acute myelogenous leukemia
  • calcitonin gene
  • polymerase chain reaction

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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