Use of the polymerase chain reaction to detect hypermethylation in the calcitonin gene. A new, sensitive approach to monitor tumor cells in acute myelogenous leukemia

Takashi Fukuhara, W. Craig Hooper, Stephen B Baylin, Jane Benson, Janet Pruckler, Anita C. Olson, Bruce L. Evatt, William R. Vogler

Research output: Contribution to journalArticle

Abstract

Based an the recent observations that, in a majority of patients with acute leukemia, the 5′ end of the calcitonin gene was hypermethylated and abnormal DNA fragments were observed following HpaII restriction digestion, we have developed a PCR-based method to sensitively detect this abnormal methylation of the calcitonin gene in AML. Applying the concept of competitive PCR, a semi-quantitative correlation was obtained between the amount of hypermethylation and the amount of leukemic cells present. These results suggest that this method will be useful to monitor the amount of tumor cells in bone marrow from patients with AML.

Original languageEnglish (US)
Pages (from-to)1031-1040
Number of pages10
JournalLeukemia Research
Volume16
Issue number10
DOIs
StatePublished - 1992

Fingerprint

Calcitonin
Acute Myeloid Leukemia
Polymerase Chain Reaction
Bone Marrow Cells
Methylation
Genes
Digestion
Neoplasms
Leukemia
DNA

Keywords

  • acute myelogenous leukemia
  • calcitonin gene
  • HL-60
  • HpaII
  • Hypermethylation
  • polymerase chain reaction
  • Southern-blot analysis

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Use of the polymerase chain reaction to detect hypermethylation in the calcitonin gene. A new, sensitive approach to monitor tumor cells in acute myelogenous leukemia. / Fukuhara, Takashi; Hooper, W. Craig; Baylin, Stephen B; Benson, Jane; Pruckler, Janet; Olson, Anita C.; Evatt, Bruce L.; Vogler, William R.

In: Leukemia Research, Vol. 16, No. 10, 1992, p. 1031-1040.

Research output: Contribution to journalArticle

Fukuhara, Takashi ; Hooper, W. Craig ; Baylin, Stephen B ; Benson, Jane ; Pruckler, Janet ; Olson, Anita C. ; Evatt, Bruce L. ; Vogler, William R. / Use of the polymerase chain reaction to detect hypermethylation in the calcitonin gene. A new, sensitive approach to monitor tumor cells in acute myelogenous leukemia. In: Leukemia Research. 1992 ; Vol. 16, No. 10. pp. 1031-1040.
@article{71726374d82440c2b63a1ca69ecf857b,
title = "Use of the polymerase chain reaction to detect hypermethylation in the calcitonin gene. A new, sensitive approach to monitor tumor cells in acute myelogenous leukemia",
abstract = "Based an the recent observations that, in a majority of patients with acute leukemia, the 5′ end of the calcitonin gene was hypermethylated and abnormal DNA fragments were observed following HpaII restriction digestion, we have developed a PCR-based method to sensitively detect this abnormal methylation of the calcitonin gene in AML. Applying the concept of competitive PCR, a semi-quantitative correlation was obtained between the amount of hypermethylation and the amount of leukemic cells present. These results suggest that this method will be useful to monitor the amount of tumor cells in bone marrow from patients with AML.",
keywords = "acute myelogenous leukemia, calcitonin gene, HL-60, HpaII, Hypermethylation, polymerase chain reaction, Southern-blot analysis",
author = "Takashi Fukuhara and Hooper, {W. Craig} and Baylin, {Stephen B} and Jane Benson and Janet Pruckler and Olson, {Anita C.} and Evatt, {Bruce L.} and Vogler, {William R.}",
year = "1992",
doi = "10.1016/0145-2126(92)90083-J",
language = "English (US)",
volume = "16",
pages = "1031--1040",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier Limited",
number = "10",

}

TY - JOUR

T1 - Use of the polymerase chain reaction to detect hypermethylation in the calcitonin gene. A new, sensitive approach to monitor tumor cells in acute myelogenous leukemia

AU - Fukuhara, Takashi

AU - Hooper, W. Craig

AU - Baylin, Stephen B

AU - Benson, Jane

AU - Pruckler, Janet

AU - Olson, Anita C.

AU - Evatt, Bruce L.

AU - Vogler, William R.

PY - 1992

Y1 - 1992

N2 - Based an the recent observations that, in a majority of patients with acute leukemia, the 5′ end of the calcitonin gene was hypermethylated and abnormal DNA fragments were observed following HpaII restriction digestion, we have developed a PCR-based method to sensitively detect this abnormal methylation of the calcitonin gene in AML. Applying the concept of competitive PCR, a semi-quantitative correlation was obtained between the amount of hypermethylation and the amount of leukemic cells present. These results suggest that this method will be useful to monitor the amount of tumor cells in bone marrow from patients with AML.

AB - Based an the recent observations that, in a majority of patients with acute leukemia, the 5′ end of the calcitonin gene was hypermethylated and abnormal DNA fragments were observed following HpaII restriction digestion, we have developed a PCR-based method to sensitively detect this abnormal methylation of the calcitonin gene in AML. Applying the concept of competitive PCR, a semi-quantitative correlation was obtained between the amount of hypermethylation and the amount of leukemic cells present. These results suggest that this method will be useful to monitor the amount of tumor cells in bone marrow from patients with AML.

KW - acute myelogenous leukemia

KW - calcitonin gene

KW - HL-60

KW - HpaII

KW - Hypermethylation

KW - polymerase chain reaction

KW - Southern-blot analysis

UR - http://www.scopus.com/inward/record.url?scp=0026801042&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026801042&partnerID=8YFLogxK

U2 - 10.1016/0145-2126(92)90083-J

DO - 10.1016/0145-2126(92)90083-J

M3 - Article

VL - 16

SP - 1031

EP - 1040

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

IS - 10

ER -