The degree of inhibition of respiration-dependent vs respiration-independent Ca2+ binding by rat liver mitochondria by different members of the lanthanide family was used to establish the existence of two different classes of Ca2+ binding sites. The distinction is based on the differences in cation:site interactions between the two classes of sites and the members of the lanthanide series. Lanthanide inhibition of respiration-dependent Ca2+ uptake suggests that the binding site is specific for the calcium ion. Those members of the lanthanide family whose ionic radii are nearer that of Ca2+ are the best inhibitors. The inhibition of respiration-independent Ca2+ binding is much different, indicating non-specific cation absorption.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Sep 23 1977|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology