Use of T-cell antibodies for donor dosaging in a canine model of in utero hematopoietic stem cell transplantation

Scott M. Petersen, Mariya Gendelman, Kathleen M. Murphy, Michael Torbenson, Richard J. Jones, Janyne E. Althaus, Gail Stetten, Chris Bird, Karin J. Blakemore

Research output: Contribution to journalArticle

Abstract

Aim: Microchimerism following canine in utero hematopoietic stem cell transplantation (IUHSCT) development of T-cell dosing regimens. Objective: To investigate the use of anti-T-cell antibodies for cell dosing of the donor graft in a canine model of IUHSCT. Study Design: Canine IUHSCT was performed by ultrasound-guided intraperitoneal injection in days 35-38 of fetal canines with CD34+ cells at doses of 4.5 × 108 to 1.3 × 109 cells/kg and T cells (CD3+ CD5+) at doses of 8 × 106 to 8.8 × 108 cells/kg. Postnatal studies included tissue histology and polymerase chain reaction-based chimerism analysis. Results: Term survival was 86-100%. Microchimerism (0-2%) was detected in five of eight recipients in multiple tissues. Histopathology revealed no evidence of graft-versus-host disease (GVHD). Conclusion: Canine IUHSCT is a useful model to investigate the role of donor T cells in engraftment and GVHD. IUHSCT at early gestational ages with high doses of donor T cells in the graft yields microchimerism in multiple tissues without GVHD.

Original languageEnglish (US)
Pages (from-to)175-179
Number of pages5
JournalFetal Diagnosis and Therapy
Volume22
Issue number3
DOIs
StatePublished - Apr 1 2007

Keywords

  • Bone marrow transplantation
  • Canine model
  • Hematopoietic stem cell

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Embryology
  • Radiology Nuclear Medicine and imaging
  • Obstetrics and Gynecology

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