Use of RAPD-PGR to identify true hybrid plants from crosses between closely related progenitors

Ana Lilia Alzate-Marin, Gilson Soares Baía, Sebastião Martins Filho, Trazilbo José De Paula, Carlos Sigueyuki Sediyama, Everaldo Gonçalves De Barros, Maurilio Alves Moreira

Research output: Contribution to journalArticle

Abstract

RAPD-PCR molecular markers were used to identify common bean and soybean hybrid plants derived from crosses between closely related progenitors, with no apparent phenotypic differences. Primers OP-F12 and OP-C03 were used to identify true hybrids derived from crosses between common bean cultivars Ruda (A 285) and AN 910408, and soybean cultivars Cristalina and Bossier, respectively. Each primer generated one polymorphic DNA band which was present in the male progenitor and absent in the female progenitor. As RAPD bands are normally inherited as dominant characters, the presence of these bands in the Fi plants confirmed their status.

Original languageEnglish (US)
Pages (from-to)621-623
Number of pages3
JournalBrazilian Journal of Genetics
Volume19
Issue number4
DOIs
StatePublished - Dec 1996

ASJC Scopus subject areas

  • Genetics

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    Alzate-Marin, A. L., Baía, G. S., Martins Filho, S., De Paula, T. J., Sediyama, C. S., De Barros, E. G., & Moreira, M. A. (1996). Use of RAPD-PGR to identify true hybrid plants from crosses between closely related progenitors. Brazilian Journal of Genetics, 19(4), 621-623. https://doi.org/10.1590/S0100-84551996000400016