Use of ProteinChip technology for identifying biomarkers of parasitic diseases: The example of porcine cysticercosis (Taenia solium)

N. Deckers, P. Dorny, K. Kanobana, J. Vercruysse, A. E. Gonzalez, B. Ward, M. Ndao

Research output: Contribution to journalArticlepeer-review

Abstract

Taenia solium cysticercosis is a significant public health problem in endemic countries. The current serodiagnostic techniques are not able to differentiate between infections with viable cysts and infections with degenerated cysts. The objectives of this study were to identify specific novel biomarkers of these different disease stages in the serum of experimentally infected pigs using ProteinChip technology (Bio-Rad) and to validate these biomarkers by analyzing serum samples from naturally infected pigs. In the experimental sample set 30 discriminating biomarkers (p < 0.05) were found, 13 specific for the viable phenotype, 9 specific for the degenerated phenotype and 8 specific for the infected phenotype (either viable or degenerated cysts). Only 3 of these biomarkers were also significant in the field samples; however, the peak profiles were not consistent among the two sample sets. Five biomarkers discovered in the sera from experimentally infected pigs were identified as clusterin, lecithin-cholesterol acyltransferase, vitronectin, haptoglobin and apolipoprotein A-I.

Original languageEnglish (US)
Pages (from-to)320-329
Number of pages10
JournalExperimental Parasitology
Volume120
Issue number4
DOIs
StatePublished - Dec 2008

Keywords

  • 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate
  • Apo A-I
  • Apo A-IV
  • Biological markers
  • CHAPS
  • CV
  • Cestode
  • Cysticercosis
  • DTT
  • EDM
  • HDL
  • IEF
  • IMAC
  • IgG
  • LCAT
  • MW
  • Pig
  • QC
  • S/N
  • SDS
  • SELDI-TOF MS
  • Surface Enhanced Laser Desorption-Ionization Time of Flight (SELDI-TOF)
  • Taenia solium
  • WCX
  • apolipoprotein A-I
  • apolipoprotein A-IV
  • coefficient of variation
  • dithiothreitol
  • expression difference mapping
  • high density lipoprotein
  • immobilized metal affinity capture
  • immunoglobulin G
  • isoelectric focusing
  • lecithin-cholesterol acyltransferase
  • m/z
  • mass to charge ratio
  • molecular weight
  • quality control
  • signal to noise ratio
  • sodium dodecyl sulfate
  • surface enhanced laser desorption-ionization time-of-flight mass spectrometry
  • weak cation exchange

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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