Use of PGMY primers in L1 consensus PCR improves detection of human papillomavirus DNA in genital samples

François Coutlée, Patti Gravitt, Janet Kornegay, Catherine Hankins, Harriet Richardson, Normand Lapointe, Hélène Voyer, Eduardo Franco

Research output: Contribution to journalArticle

Abstract

The novel PGMY L1 consensus primer pair is more sensitive than the MY09 and MY11 primer mix for detection and typing with PCR of human papillomavirus (HPV) DNA in genital specimens. We assessed the diagnostic yield of PGMY primers for the detection and typing of HPV by comparing the results obtained with PGMY09/PGMY11 and MY09/MY11/HMB01 on 299 genital samples. Amplicons generated with PGMY primers were typed with the line blot assay (PGMY-line blot), while HPV amplicons obtained with the degenerate primer pool MY09/MY11/HMB01 were detected with type-specific radiolabeled probes in a dot blot assay (standard consensus PCR test). Cervicovaginal lavage samples (N = 272) and cervical scrape samples (N = 27) were tested in parallel with both PCR tests. The PGMY-line blot test detected the presence of HPV DNA more frequently than the standard consensus PCR assay. The concordance for HPV typing between the two assays was 84.3% (214 of 255 samples), for a good kappa value of 0.69. Of the 177 samples containing HPV DNA by at least one method, 40 samples contained at least one HPV type detected only with PGMY-line blot, whereas positivity exclusively with the standard consensus PCR test was found for only 7 samples (P <0.001). HPV types 45 and 52 were especially more frequently detected with PGMY than MY primers. However, most HPV types were better amplified with PGMY primers, including HPV-16. Samples with discordant results between the two PCR assays more frequently contained multiple HPV types. Studies using PGMY instead of MY primers have the potential to report higher detection rates of HPV infection not only for newer HPV types but also for well-known genital types.

Original languageEnglish (US)
Pages (from-to)902-907
Number of pages6
JournalJournal of Clinical Microbiology
Volume40
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

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Polymerase Chain Reaction
DNA
Papillomavirus Infections
Human papillomavirus 16
Therapeutic Irrigation

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Coutlée, F., Gravitt, P., Kornegay, J., Hankins, C., Richardson, H., Lapointe, N., ... Franco, E. (2002). Use of PGMY primers in L1 consensus PCR improves detection of human papillomavirus DNA in genital samples. Journal of Clinical Microbiology, 40(3), 902-907. https://doi.org/10.1128/JCM.40.3.902-907.2002

Use of PGMY primers in L1 consensus PCR improves detection of human papillomavirus DNA in genital samples. / Coutlée, François; Gravitt, Patti; Kornegay, Janet; Hankins, Catherine; Richardson, Harriet; Lapointe, Normand; Voyer, Hélène; Franco, Eduardo.

In: Journal of Clinical Microbiology, Vol. 40, No. 3, 2002, p. 902-907.

Research output: Contribution to journalArticle

Coutlée, F, Gravitt, P, Kornegay, J, Hankins, C, Richardson, H, Lapointe, N, Voyer, H & Franco, E 2002, 'Use of PGMY primers in L1 consensus PCR improves detection of human papillomavirus DNA in genital samples', Journal of Clinical Microbiology, vol. 40, no. 3, pp. 902-907. https://doi.org/10.1128/JCM.40.3.902-907.2002
Coutlée, François ; Gravitt, Patti ; Kornegay, Janet ; Hankins, Catherine ; Richardson, Harriet ; Lapointe, Normand ; Voyer, Hélène ; Franco, Eduardo. / Use of PGMY primers in L1 consensus PCR improves detection of human papillomavirus DNA in genital samples. In: Journal of Clinical Microbiology. 2002 ; Vol. 40, No. 3. pp. 902-907.
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