Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species

Megan E. Reller, Amanda B. Mallonee, Nicole P. Kwiatkowski, William G. Merz

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently.

Original languageEnglish (US)
Pages (from-to)3802-3803
Number of pages2
JournalJournal of clinical microbiology
Volume45
Issue number11
DOIs
StatePublished - Nov 2007

ASJC Scopus subject areas

  • Microbiology (medical)

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