Use of identical assay conditions for cocaine analog binding and dopamine uptake to identify potential cocaine antagonists

Barbara S. Slusher, Carol W. Tiffany, Jennifer L. Olkowski, Paul F. Jackson

Research output: Contribution to journalArticle

Abstract

The addictive and euphorogenic properties of cocaine are thought to result from inhibition of the dopamine transporter (DAT). Recent evidence suggests that dopamine and cocaine bind to distinct sites on the transporter protein. Therefore it should be possible to design drugs which specifically inhibit cocaine recognition by the DAT while permitting the transporter to maintain its function of accumulating dopamine. One way to monitor such activity is to compare the inhibition constants of test agents for inhibition of radiolabelled dopamine uptake (K(i(uptake))) and inhibition of the binding of a cocaine ligand such as [3H]2β-carbomethoxy-3β-(fluorophenyl)tropane (CFT; K(i(bind))) and select for compounds with K(i(uptake))/K(i(bind)) ratios greater than unity. Because others have shown that compounds can exhibit K(i(uptake))/K(i(bind)) ratios greater than unity when the assays are performed under non-identical conditions, we have established these assays under identical conditions of time, temperature and buffer using a Chinese hamster ovary (CHO) cell line which stably expresses the human DAT. Kinetic and saturation analyses were performed on both assays and over 200 structurally diverse compounds were screened. Using identical assay parameters, several series of compounds having K(i(uptake))/K(i(bind)) ratios significantly greater than unity were identified. Such compounds include local anesthetics (procaine, dibucaine, tolperisone, dyclonine, diperodone)l antipsychotic agents (10-(diethylaminopropionyl)phenothiazin antidepressants (desipramine, imipramine, protriptyline), a diuretic (5-N-methyl-N-isobutyl-amilioride), an anticholinergic agent (prindinol), a PKC inhibitor (H-8), a calcium channel antagonist (loperamide) and an antimalarial compound (chloroquine). To our knowledge, even though these compounds exhibit low binding affinities (3-24 μM), they represent some of the most cocaine site-selective compounds identified to date using identical assay parameters.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalDrug and alcohol dependence
Volume48
Issue number1
DOIs
StatePublished - Oct 25 1997

Keywords

  • CHO cells
  • Cocaine
  • Cocaine antagonists
  • Dopamine transporter
  • [H]2β-carbomethoxy-3β-(fluorophenyl)tropane

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Psychiatry and Mental health
  • Pharmacology (medical)

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