Use of human perivascular stem cells for bone regeneration

Aaron W. James, Janette N. Zara, Mirko Corselli, Michael Chiang, Wei Yuan, Virginia Nguyen, Asal Askarinam, Raghav Goyal, Ronald K. Siu, Victoria Scott, Min Lee, Kang Ting, Bruno Péault, Chia Soo

Research output: Contribution to journalArticlepeer-review

Abstract

Human perivascular stem cells (PSCs) can be isolated in sufficient numbers from multiple tissues for purposes of skeletal tissue engineering 1-3 . PSCs are a FACS-sorted population of 'pericytes' (CD146+CD45-CD45-) and 'adventitial cells' (CD146-CD34+CD45-), each of which we have previously reported to have properties of mesenchymal stem cells. PSCs, like MSCs, are able to undergo osteogenic differentiation, as well as secrete pro-osteogenic cytokines 1,2 . In the present protocol, we demonstrate the osteogenicity of PSCs in several animal models including a muscle pouch implantation in SCID (severe combined immunodeficient) mice, a SCID mouse calvarial efect and a femoral segmental defect (FSD) in athymic rats. The thigh muscle pouch model is used to assess ectopic bone formation. Calvarial defects are centered on the parietal bone and are standardly 4 mm in diameter (critically sized) 8 . FSDs are bicortical and are stabilized with a polyethylene bar and K-wires 4 . The FSD described is also a critical size defect, which does not significantly heal on its own 4 . In contrast, if stem cells or growth factors are added to the defect site, significant bone regeneration can be appreciated. The overall goal of PSC xenografting is to demonstrate the osteogenic capability of this cell type in both ectopic and orthotopic bone regeneration models.

Original languageEnglish (US)
Article numbere2952
JournalJournal of Visualized Experiments
Issue number63
DOIs
StatePublished - May 25 2012

Keywords

  • Bioengineering
  • Biomedical engineering
  • Bone defect
  • Calvarial defect
  • Femoral defect
  • Osteogenesis
  • Pericyte
  • Stem cell
  • Stem cell biology
  • Tissue engineering

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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