Use of Formamide in Nucleic Acid Reassociation

B. J. Schmeckpeper; Kirby D. Smith

doi:10.1021/bi00757a032

Use of Formamide in Nucleic Acid Reassociation

B. J. Schmeckpeper, Kirby D. Smith

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The effect of formamide on DNA-RNA hybridization has been examined by directly comparing the thermal stabilities of the duplexes formed at various criteria. Although increasing formamide concentrations in the reassociation buffers elevate the mean thermal stabilities of the hybrids, there is no simple linear relationship between the mean ther- mal stabilities of hybrids and the formamide concentrations. Also, there is no simple linear relationship between the formamide concentration necessary to produce hybrids of specified thermal stability and the temperature of hybrid formation. Finally, we have noted a large decrease in the rate of reaction under some conditions.

Original language	English (US)
Pages (from-to)	1319-1326
Number of pages	8
Journal	Biochemistry
Volume	11
Issue number	7
DOIs	https://doi.org/10.1021/bi00757a032
State	Published - Mar 1 1972
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

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AB - The effect of formamide on DNA-RNA hybridization has been examined by directly comparing the thermal stabilities of the duplexes formed at various criteria. Although increasing formamide concentrations in the reassociation buffers elevate the mean thermal stabilities of the hybrids, there is no simple linear relationship between the mean ther- mal stabilities of hybrids and the formamide concentrations. Also, there is no simple linear relationship between the formamide concentration necessary to produce hybrids of specified thermal stability and the temperature of hybrid formation. Finally, we have noted a large decrease in the rate of reaction under some conditions.

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Use of Formamide in Nucleic Acid Reassociation

Abstract

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