Use of formamide in nucleic acid reassociation

B. J. Schmeckpeper, Kirby D. Smith

Research output: Contribution to journalArticle

Abstract

The effect of formamide on DNA-RNA hybridization has been examined by directly comparing the thermal stabilities of the duplexes formed at various criteria. Although increasing formamide concentrations in the reassociation buffers elevate the mean thermal stabilities of the hybrids, there is no simple linear relationship between the mean thermal stabilities of hybrids and the formamide concentrations. Also, there is no simple linear relationship between the formamide concentration necessary to produce hybrids of specified thermal stability and the temperature of hybrid formation. Finally, we have noted a large decrease in the rate of reaction under some conditions.

Original languageEnglish (US)
Pages (from-to)1319-1326
Number of pages8
JournalBiochemistry®
Volume11
Issue number7
StatePublished - 1972

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Nucleic Acids
Thermodynamic stability
Hot Temperature
Buffers
RNA
Temperature
formamide
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Use of formamide in nucleic acid reassociation. / Schmeckpeper, B. J.; Smith, Kirby D.

In: Biochemistry®, Vol. 11, No. 7, 1972, p. 1319-1326.

Research output: Contribution to journalArticle

Schmeckpeper, BJ & Smith, KD 1972, 'Use of formamide in nucleic acid reassociation', Biochemistry®, vol. 11, no. 7, pp. 1319-1326.
Schmeckpeper, B. J. ; Smith, Kirby D. / Use of formamide in nucleic acid reassociation. In: Biochemistry®. 1972 ; Vol. 11, No. 7. pp. 1319-1326.
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