Use of Formamide in Nucleic Acid Reassociation

B. J. Schmeckpeper, Kirby D. Smith

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Abstract

The effect of formamide on DNA-RNA hybridization has been examined by directly comparing the thermal stabilities of the duplexes formed at various criteria. Although increasing formamide concentrations in the reassociation buffers elevate the mean thermal stabilities of the hybrids, there is no simple linear relationship between the mean ther- mal stabilities of hybrids and the formamide concentrations. Also, there is no simple linear relationship between the formamide concentration necessary to produce hybrids of specified thermal stability and the temperature of hybrid formation. Finally, we have noted a large decrease in the rate of reaction under some conditions.

Original languageEnglish (US)
Pages (from-to)1319-1326
Number of pages8
JournalBiochemistry
Volume11
Issue number7
DOIs
StatePublished - Mar 1 1972

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ASJC Scopus subject areas

  • Biochemistry

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