Use of antibodies in lymphocyte secretions for detection of subclinical tuberculosis infection in asymptomatic contacts

Rubhana Raqib, S. M.Mostafa Kamal, M. Jubayer Rahman, Zeaur Rahim, Sayera Banu, Pradip K. Bardhan, Fahima Chowdhury, Gul Ara, K. Zaman, Robert F. Breiman, Jan Andersson, David A. Sack

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously demonstrated that Mycobacterium bovis BCG-specific inimunoglobulin G antibodies in lymphocyte secretions (ALS) can be employed as a marker for active tuberculosis (TB). We aimed to determine whether the ALS method allows detection of subclinical TB infection in asymptomatic individuals. A prospective study of family contacts (FCs) of patients with active TB and healthy controls was performed. Thirteen of 42 FCs had high ALS responses, including 6 FCs who subsequently developed active TB. No correlation was observed between the tuberculin skin test and the ALS responses in the FCs (r = 0.1, P = 0.23). Among patients with active TB, BCG-speciflc ALS responses steadily declined from the time of diagnosis through 6 months following antimycobacterial chemotherapy (P = 0.001). The ALS assay enabled detection of infection in exposed symptom-free contacts, who are at greater risk for developing active TB. The method may also allow discrimination between effective treatment of active infection and suboptimal response to therapy.

Original languageEnglish (US)
Pages (from-to)1022-1027
Number of pages6
JournalClinical and Diagnostic Laboratory Immunology
Volume11
Issue number6
DOIs
StatePublished - Nov 2004
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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