Use of an enzyme immunoassay to detect serum IgG antibodies to Haemophilus ducreyi

K. Museyi, E. Van Dyck, T. Vervoort, D. Taylor, C. Hoge, P. Piot

Research output: Contribution to journalArticle

Abstract

An experimental enzyme immunoassay (EIA) for detecting serum IgG antibody to Haemophilus ducreyi was developed using an ultrasonicated whole-cell antigen. The mean optical densities (OD) for sera from men with proven chancroid from Nairobi (47 patients) and Bangkok (72 patients) were significantly higher than those obtained from Nairobi men with genital ulcers not due to H. ducreyi, from Nairobi men with urethritis, from pregnant women in Nairobi, and from European men with sexually transmitted disease. When an OD of 0.500 was taken as the cutoff value, 89% and 55% of men with proven chancroid in Nairobi and Bangkok, respectively, were positive for H. ducreyi antibody, as compared with 2%-17% in the control groups. A rise in OD was observed in five of 18 patients with clinical chancroid. These results confirm the development of circulating antibodies in chancroid and suggest that this EIA may be useful for the diagnosis and epidemiological study of H. ducreyi infection.

Original languageEnglish (US)
Pages (from-to)1039-1043
Number of pages5
JournalJournal of Infectious Diseases
Volume157
Issue number5
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Museyi, K., Van Dyck, E., Vervoort, T., Taylor, D., Hoge, C., & Piot, P. (1988). Use of an enzyme immunoassay to detect serum IgG antibodies to Haemophilus ducreyi. Journal of Infectious Diseases, 157(5), 1039-1043. https://doi.org/10.1093/infdis/157.5.1039