Use of acousto-optic tuneable filter in fluorescence imaging endoscopy

Mounir Bouhifd, Maurice Whelan, Marc Aprahamian

Research output: Contribution to journalConference articlepeer-review


A prototype instrument for fluorescence-based medical diagnostics in vivo is described. The system consists of a rigid endoscope comprising a UV laser-source for fluorescence excitation and a white light source for direct imaging. An acousto-optic tuneable filter (AOTF) is employed as a full-field tuneable bandpass filter. This allows fast continuous or random-access tuning with high filtering efficiency. A study of the diagnostic potential of fluorescence imaging for pancreatitis was conducted on a rat model. In particular, the aim was to detect autofluorescence of endogenous protoporphyrin IX (PpIX) that has been shown to accumulate in early-stage diseased tissue undergoing an inflammatory response.

Original languageEnglish (US)
Pages (from-to)305-314
Number of pages10
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Dec 1 2003
EventNovel Optical Instrumentation for Biomedical Applications - Munich, Germany
Duration: Jun 24 2003Jun 25 2003


  • AOTF filtering
  • Endogenous protoporphyrin IX
  • Endoscope
  • Fluorescence imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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