Use of a single slide trichrome-stained concentrate for the detection of intestinal parasites: Stained concentration procedure for ova and parasites

Devon C. Hale, Karen C Carroll, James R. Kucera, William E. Aldeen

Research output: Contribution to journalArticle

Abstract

The detection and identification of intestinal parasites was studied using a single slide of a trichrome-stained concentrate made from a cupric- polyvinyl alcohol (PVA)-preserved specimen. When saline was used in place of formalin in the concentration procedure, it was possible to adequately stain the concentrate with a modified-trichrome stain. This was compared to a more traditional two-slide ova and parasite (OandP) procedure. Of the 1,000 specimens evaluated, a total of 261 parasites were detected using the single slide, modified trichrome-stained concentrate compared to 249 from the formalin/Hemo-De wet mount and 216 from the unconcentrated trichrome stain. The detection rate of the single slide method was comparable to the combined detection rate of the two-slide method. The saline/Hemo-De concentration procedure has the advantage of using nontoxic saline in place of formaldehyde. This new method is more cost-effective because it requires the processing and evaluation of only one slide.

Original languageEnglish (US)
Pages (from-to)175-179
Number of pages5
JournalAmerican Journal of Clinical Pathology
Volume106
Issue number2
StatePublished - Aug 1996
Externally publishedYes

Fingerprint

Ovum
Parasites
Formaldehyde
Polyvinyl Alcohol
Coloring Agents
Costs and Cost Analysis
trichrome stain
Hemo-De

Keywords

  • Ova and parasite procedure
  • Trichrome-stained concentrate

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Use of a single slide trichrome-stained concentrate for the detection of intestinal parasites : Stained concentration procedure for ova and parasites. / Hale, Devon C.; Carroll, Karen C; Kucera, James R.; Aldeen, William E.

In: American Journal of Clinical Pathology, Vol. 106, No. 2, 08.1996, p. 175-179.

Research output: Contribution to journalArticle

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