Use of a ROSA26: GFP transgenic line for long-term Xenopus fate-mapping studies

Joshua B. Gross, James Hanken, Ericka Oglesby, Nicholas Marsh-Armstrong

Research output: Contribution to journalArticle

Abstract

Widespread and persistent marker expression is a prerequisite for many transgenic applications, including chimeric transplantation studies. Although existing transgenic tools for the clawed frog, Xenopus laevis, offer a number of promoters that drive widespread expression during embryonic stages, obtaining transgene expression through metamorphosis and into differentiated adult tissues has been difficult to achieve with this species. Here we report the application of the murine ROSA26 promoter in Xenopus. GFP is expressed in every transgenic tissue and cell type examined at post-metamorphic stages. Furthermore, transgenic ROSA26:GFP frogs develop normally, with no apparent differences in growth or morphology relative to wild-type frogs. ROSA26 transgenes may be used as a reliable marker for embryonic fate-mapping of adult structures in Xenopus laevis. Utility of this transgenic line is illustrated by its use in a chimeric grafting study that demonstrates the derivation of the adult bony jaw from embryonic cranial neural crest.

Original languageEnglish (US)
Pages (from-to)401-413
Number of pages13
JournalJournal of Anatomy
Volume209
Issue number3
DOIs
StatePublished - Sep 2006

Fingerprint

Xenopus
frog
Anura
Xenopus laevis
genetically modified organisms
Transgenes
frogs
Neural Crest
transplantation
transgenes
Jaw
metamorphosis
promoter regions
Transplantation
neural crest
jaws
Growth
mice
tissue
marker

Keywords

  • Bone
  • Chimera
  • Cranial neural crest
  • Developmental biology
  • Skull

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Use of a ROSA26 : GFP transgenic line for long-term Xenopus fate-mapping studies. / Gross, Joshua B.; Hanken, James; Oglesby, Ericka; Marsh-Armstrong, Nicholas.

In: Journal of Anatomy, Vol. 209, No. 3, 09.2006, p. 401-413.

Research output: Contribution to journalArticle

Gross, Joshua B. ; Hanken, James ; Oglesby, Ericka ; Marsh-Armstrong, Nicholas. / Use of a ROSA26 : GFP transgenic line for long-term Xenopus fate-mapping studies. In: Journal of Anatomy. 2006 ; Vol. 209, No. 3. pp. 401-413.
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