Abstract
Neisseria gonorrhoeae is the most common sexually transmitted disease-causing bacterium worldwide. An in-house PCR assay targeting the carbamoyl-phosphate synthase subunit A (carA) gene was developed for the specific detection of N. gonorrhoeae in clinical specimens. Samples from 605 patients were cultured on selective medium and assayed by PCR in a double-blind fashion. Of 605 urethral/cervical samples analysed, 13 were PCR-positive, of which 11 were culture-positive. The PCR showed a sensitivity and specificity of 100% and 99.7% with these samples. PCR targeting the carA gene appears to be a reliable method for the detection of N. gonorrhoeae in clinical specimens.
Original language | English (US) |
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Pages (from-to) | 809-812 |
Number of pages | 4 |
Journal | Clinical Microbiology and Infection |
Volume | 12 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2006 |
Keywords
- Diagnosis
- Neisseria gonorrhoeae
- PCR
- Sexually transmitted disease
- Swab samples
- carA gene
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases