Use of a monoclonal antibody in an enzyme immunoassay for the detection of Entamoeba histolytica in fecal specimens

B. L.P. Ungar, R. H. Yolken, T. C. Quinn

Research output: Contribution to journalArticlepeer-review

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Entamoeba histolytica in human feces, using both a monoclonal antibody and rabbit antisera. It detected from less than 1 to 57 trophozoites of 6 E. histolytica strains. Stool specimens were positive by ELISA in 18 of 22 (82%) patients with E. histolytica and in 3 of 186 (2%) of patients without demonstrable E. histolytica in their stools. The latter included one from a child living near an asymptomatic cyst carrier and another from a traveler with giardiasis who had recently taken antibiotics. One hundred eight of 183 microscopy-and ELISA-negative specimens contained other parasites including Giardia (49 specimens), Endolimax nana (24), Entamoeba coli (21), Iodamoeba butschlii (2), and Entamoeba hartmanni (1). This ELISA for E. histolytica is a simple, sensitive and specific diagnostic tool.

Original languageEnglish (US)
Pages (from-to)465-472
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume34
Issue number3
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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