Use of a modified bacteriophage to probe the interactions between peptides and ion channel receptors in mammalian cells

Min Li

Research output: Contribution to journalArticle

Abstract

Besides natural peptide ligands, screening of random peptide libraries has yielded novel bioactive peptides for cell surface receptors. A method is described that uses a modified bacteriophage as a detection reagent to monitor the expression of receptor channels in mammalian cells and to probe the molecular interaction between phage-tethered peptides (ΦT-peptides) and specific receptor targets. By taking advantage of a specific multivalent interaction between ΦT-peptides and the receptor target, assays have been developed that use ΦT-peptides specific for the N-methyl-D-aspartate glutamate receptor, an important ligand-gated ion channel in the nervous system, to monitor the receptor expression in cultured mammalian cells. Combining these ΦT-peptide binding assays with fluorescence-activated cell sorting, 104 random glutamate receptor mutants were screened and candidate interaction residues were identified. This dual heterologous expression system offers a powerful approach to the molecular studies of protein-protein interactions.

Original languageEnglish (US)
Pages (from-to)559-563
Number of pages5
JournalNature biotechnology
Volume15
Issue number6
DOIs
StatePublished - Jun 1997

Keywords

  • NMDA receptors
  • Random peptide display
  • Receptor detection

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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