Use of a macrophage-tropic GFP-tagged human immunodeficiency virus type 1 (HIV-1) to study viral reservoirs

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The human immunodeficiency virus type 1 (HIV-1) predominantly infects two main cell types: T-lymphocytes and monocyte-derived cells such as macrophages, dendritic cells, and Langerhans cells. Studies in patients aimed at uncovering and understanding the molecular mechanisms for viral persistence and latency in macrophages in particular are challenging because these cells reside in tissues and can only be extracted using invasive methods. The in vitro human monocyte-derived macrophage (MDM) model provides an amenable system to study HIV-host cell interactions at the molecular level. Infection of macrophages with a recombinant M-tropic virus that contains the green fluorescent protein (GFP) gene within its genome allows the detection of cells with integrated viral DNA that are producing viral particles. The infected cells can be studied at the single-cell level using a variety of fluorescence-based technologies such as flow cytometry, conventional and confocal microscopy, and laser capture microdissection.

Original languageEnglish (US)
Title of host publicationViral Applications of Green Fluorescent Protein
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages165-175
Number of pages11
ISBN (Print)9781934115879
DOIs
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
Volume515
ISSN (Print)1064-3745

Keywords

  • Flow cytometry
  • Green fluorescent protein
  • HIV-1
  • Human immunodeficiency virus type 1
  • Laser capture microdissection
  • Monocyte-derived macrophages

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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