Use of a hybrid capture assay of self-collected vaginal swabs in rural Uganda for detection of human papillomavirus

David Serwadda, Maria J. Wawer, Keerti V. Shah, Nelson K. Sewankambo, Richard Daniel, Chuanjun Li, Attila Lorincz, Mary P. Meehan, Fred Wabwire-Mangen, Ronald H. Gray

Research output: Contribution to journalArticle

Abstract

A random sample of 960 women aged 15-59 years enrolled in a population- based study in rural Uganda were asked to provide self-collected vaginal swabs for human papillomavirus (HPV) testing by hybrid capture assay. The intensity of HPV infection was assessed by the relative light unit (RLU) ratio in the specimen-to-positive control (PC) ratio. In total, 898 women (93%) provided a swab and 737 provided serum for human immunodeficiency virus type 1 (HIV-1) determination. HPV prevalence was 16.7% and was highest in younger women. HIV-1 prevalence was 17.8%. HPV prevalence was 44.3% in HIV- positive and 10.2% in HIV-negative women (rate ratio, 5.36; 95% confidence interval, 3.81-7.54). The intensity of HPV infection was significantly greater among HIV-positive than HIV-negative women (54.4 vs. 11.1 RLU/PC; P= .026); intensity of infection was highest in women aged <30 years. The higher prevalence and intensity of HPV infection in HIV-positive women could facilitate HPV transmission in this population. Self-collected vaginal swabs could be used in population-based screening to identify women at high risk of cervical neoplasia.

Original languageEnglish (US)
Pages (from-to)1316-1319
Number of pages4
JournalJournal of Infectious Diseases
Volume180
Issue number4
DOIs
StatePublished - Dec 1 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Serwadda, D., Wawer, M. J., Shah, K. V., Sewankambo, N. K., Daniel, R., Li, C., Lorincz, A., Meehan, M. P., Wabwire-Mangen, F., & Gray, R. H. (1999). Use of a hybrid capture assay of self-collected vaginal swabs in rural Uganda for detection of human papillomavirus. Journal of Infectious Diseases, 180(4), 1316-1319. https://doi.org/10.1086/315026