Use of a chondroitin sulfate bioadhesive to enhance integration of bioglass particles for repairing critical-size bone defects

Shuqing Yang, Qiongyu Guo, Lucas S. Shores, Ahmed Aly, Meera Ramakrishnan, Ga Hye Kim, Qiaozhi Lu, Lixin Su, Jennifer H. Elisseeff

Research output: Contribution to journalArticlepeer-review

Abstract

Replacement of autogenous or allograft bones by artificial graft materials represents a growing area of interest in current bone repair strategies. Bioactive ceramics in particulate form, such as Bioglass® (BG) 45S5, stimulate bone mineralization comparable to autologous bone grafts, but have potential issues of particle migration and inflammation. The aim of this study was to employ a chondroitin sulfate- (CS-) based bioadhesive to improve integration of the bioglass (NovaBone® Putty) to prevent particle migration and promote bone regeneration. This BG-CS composite can encapsulate bone marrow (BM) to form a mechanically stable construct, BG-CS-BM. Rheological characterization confirmed the formation of CS-BM hydrogel by reacting the CS-based bioadhesive with the BM. Compared to the bioglass, the BG-CS-BM composite demonstrated a superior capacity to maintain construct integrity under both aqueous and turbulent environments in vitro. After implantation for 4 weeks in a critical-size distal femoral bone defect in a rabbit model, there was significantly greater bone growth in BG-CS-BM as compared to bioglass-only and the empty control. Unlike BG-CS-BM, BG-CS recruited BM in situ from the bone defect. BG-CS demonstrated a similar effect in bone formation but at a comparatively slower rate than BG-CS-BM over 6-weeks' implantation.

Original languageEnglish (US)
Pages (from-to)235-242
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume103
Issue number1
DOIs
StatePublished - Jan 1 2015

Keywords

  • bioadhesive hydrogel
  • bioglass
  • bone marrow
  • bone regeneration
  • chondroitin sulfate

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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