Urine proteomic profiling for biomarkers of acute renal transplant rejection.

Shu Ling Liang, William Clarke

Research output: Contribution to journalArticle

Abstract

Acute allograft rejection is a serious impediment to long-term success in renal transplantation. Early detection of rejection is crucial for treatment of rejection, and can help avoid long-term effects such as chronic rejection or loss of the transplanted organ. The current diagnostic paradigm is a combination of clinical presentation, biochemical measurements (serum creatinine), and needle biopsy. There are significant efforts underway to find alternate biomarkers for early detection of acute rejection, including protein profiling of urine by mass spectrometry. One approach for protein profiling is to use affinity mass spectrometry - we describe a method for this using ProteinChips and SELDI-TOF mass spectrometry.

Original languageEnglish (US)
Pages (from-to)185-191
Number of pages7
JournalMethods in molecular biology (Clifton, N.J.)
Volume641
DOIs
StatePublished - 2010
Externally publishedYes

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Graft Rejection
Proteomics
Mass Spectrometry
Biomarkers
Urine
Kidney
Protein Array Analysis
Needle Biopsy
Kidney Transplantation
Allografts
Creatinine
Proteins
Serum
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Urine proteomic profiling for biomarkers of acute renal transplant rejection. / Liang, Shu Ling; Clarke, William.

In: Methods in molecular biology (Clifton, N.J.), Vol. 641, 2010, p. 185-191.

Research output: Contribution to journalArticle

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