Uranium quantification in semen by inductively coupled plasma mass spectrometry

Todor I. Todorov; John W. Ejnik; Gustavo Guandalini; Hanna Xu; Dennis Hoover; Larry Anderson; Katherine Squibb; Melissa A. McDiarmid; Jose A. Centeno

doi:10.1016/j.jtemb.2012.07.004

Uranium quantification in semen by inductively coupled plasma mass spectrometry

Todor I. Todorov, John W. Ejnik, Gustavo Guandalini, Hanna Xu, Dennis Hoover, Larry Anderson, Katherine Squibb, Melissa A. McDiarmid, Jose A. Centeno

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

In this study we report uranium analysis for human semen samples. Uranium quantification was performed by inductively coupled plasma mass spectrometry. No additives, such as chymotrypsin or bovine serum albumin, were used for semen liquefaction, as they showed significant uranium content. For method validation we spiked 2. g aliquots of pooled control semen at three different levels of uranium: low at 5. pg/g, medium at 50. pg/g, and high at 1000. pg/g. The detection limit was determined to be 0.8. pg/g uranium in human semen. The data reproduced within 1.4-7% RSD and spike recoveries were 97-100%. The uranium level of the unspiked, pooled control semen was 2.9. pg/g of semen (. n=. 10). In addition six semen samples from a cohort of Veterans exposed to depleted uranium (DU) in the 1991 Gulf War were analyzed with no knowledge of their exposure history. Uranium levels in the Veterans' semen samples ranged from undetectable (

Original language	English (US)
Pages (from-to)	2-6
Number of pages	5
Journal	Journal of Trace Elements in Medicine and Biology
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/j.jtemb.2012.07.004
State	Published - Jan 2013
Externally published	Yes

Keywords

ICP-MS
Semen
Uranium

ASJC Scopus subject areas

Biochemistry
Inorganic Chemistry
Molecular Medicine

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10.1016/j.jtemb.2012.07.004

Cite this

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title = "Uranium quantification in semen by inductively coupled plasma mass spectrometry",

abstract = "In this study we report uranium analysis for human semen samples. Uranium quantification was performed by inductively coupled plasma mass spectrometry. No additives, such as chymotrypsin or bovine serum albumin, were used for semen liquefaction, as they showed significant uranium content. For method validation we spiked 2. g aliquots of pooled control semen at three different levels of uranium: low at 5. pg/g, medium at 50. pg/g, and high at 1000. pg/g. The detection limit was determined to be 0.8. pg/g uranium in human semen. The data reproduced within 1.4-7% RSD and spike recoveries were 97-100%. The uranium level of the unspiked, pooled control semen was 2.9. pg/g of semen (. n=. 10). In addition six semen samples from a cohort of Veterans exposed to depleted uranium (DU) in the 1991 Gulf War were analyzed with no knowledge of their exposure history. Uranium levels in the Veterans' semen samples ranged from undetectable (",

keywords = "ICP-MS, Semen, Uranium",

author = "Todorov, {Todor I.} and Ejnik, {John W.} and Gustavo Guandalini and Hanna Xu and Dennis Hoover and Larry Anderson and Katherine Squibb and McDiarmid, {Melissa A.} and Centeno, {Jose A.}",

year = "2013",

month = jan,

doi = "10.1016/j.jtemb.2012.07.004",

language = "English (US)",

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AU - Todorov, Todor I.

AU - Ejnik, John W.

AU - Guandalini, Gustavo

AU - Xu, Hanna

AU - Hoover, Dennis

AU - Anderson, Larry

AU - Squibb, Katherine

AU - McDiarmid, Melissa A.

AU - Centeno, Jose A.

PY - 2013/1

Y1 - 2013/1

N2 - In this study we report uranium analysis for human semen samples. Uranium quantification was performed by inductively coupled plasma mass spectrometry. No additives, such as chymotrypsin or bovine serum albumin, were used for semen liquefaction, as they showed significant uranium content. For method validation we spiked 2. g aliquots of pooled control semen at three different levels of uranium: low at 5. pg/g, medium at 50. pg/g, and high at 1000. pg/g. The detection limit was determined to be 0.8. pg/g uranium in human semen. The data reproduced within 1.4-7% RSD and spike recoveries were 97-100%. The uranium level of the unspiked, pooled control semen was 2.9. pg/g of semen (. n=. 10). In addition six semen samples from a cohort of Veterans exposed to depleted uranium (DU) in the 1991 Gulf War were analyzed with no knowledge of their exposure history. Uranium levels in the Veterans' semen samples ranged from undetectable (

AB - In this study we report uranium analysis for human semen samples. Uranium quantification was performed by inductively coupled plasma mass spectrometry. No additives, such as chymotrypsin or bovine serum albumin, were used for semen liquefaction, as they showed significant uranium content. For method validation we spiked 2. g aliquots of pooled control semen at three different levels of uranium: low at 5. pg/g, medium at 50. pg/g, and high at 1000. pg/g. The detection limit was determined to be 0.8. pg/g uranium in human semen. The data reproduced within 1.4-7% RSD and spike recoveries were 97-100%. The uranium level of the unspiked, pooled control semen was 2.9. pg/g of semen (. n=. 10). In addition six semen samples from a cohort of Veterans exposed to depleted uranium (DU) in the 1991 Gulf War were analyzed with no knowledge of their exposure history. Uranium levels in the Veterans' semen samples ranged from undetectable (

KW - ICP-MS

KW - Semen

KW - Uranium

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Uranium quantification in semen by inductively coupled plasma mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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