Uranium quantification in semen by inductively coupled plasma mass spectrometry

Todor I. Todorov, John W. Ejnik, Gustavo Guandalini, Hanna Xu, Dennis Hoover, Larry Anderson, Katherine Squibb, Melissa A. McDiarmid, Jose A. Centeno

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


In this study we report uranium analysis for human semen samples. Uranium quantification was performed by inductively coupled plasma mass spectrometry. No additives, such as chymotrypsin or bovine serum albumin, were used for semen liquefaction, as they showed significant uranium content. For method validation we spiked 2. g aliquots of pooled control semen at three different levels of uranium: low at 5. pg/g, medium at 50. pg/g, and high at 1000. pg/g. The detection limit was determined to be 0.8. pg/g uranium in human semen. The data reproduced within 1.4-7% RSD and spike recoveries were 97-100%. The uranium level of the unspiked, pooled control semen was 2.9. pg/g of semen (. n=. 10). In addition six semen samples from a cohort of Veterans exposed to depleted uranium (DU) in the 1991 Gulf War were analyzed with no knowledge of their exposure history. Uranium levels in the Veterans' semen samples ranged from undetectable (

Original languageEnglish (US)
Pages (from-to)2-6
Number of pages5
JournalJournal of Trace Elements in Medicine and Biology
Issue number1
StatePublished - Jan 2013
Externally publishedYes


  • ICP-MS
  • Semen
  • Uranium

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry
  • Molecular Medicine


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