Uranium analysis in urine by inductively coupled plasma dynamic reaction cell mass spectrometry

John W. Ejnik, Todor I. Todorov, Florabel G. Mullick, Katherine Squibb, Melissa A. McDiarmid, Jose A. Centeno

Research output: Contribution to journalArticle

Abstract

Urine uranium concentrations are the best biological indicator for identifying exposure to depleted uranium (DU). Internal exposure to DU causes an increased amount of urine uranium and a decreased ratio of 235U/ 238U in urine samples, resulting in measurements that vary between 0.00725 and 0.002 (i.e., natural and depleted uranium's 235U/ 238U ratios, respectively). A method based on inductively coupled plasma dynamic reaction cell mass spectrometry (ICP-DRC-MS) was utilized to identify DU in urine by measuring the quantity of total U and the 235U/238U ratio. The quantitative analysis was achieved using 233U as an internal standard. The analysis was performed both with and without the reaction gas oxygen. The reaction gas converted ionized 235U+ and 238U+ into 235UO2 + (m/z=267) and 238UO 2 + (m/z=270). This conversion was determined to be over 90% efficient. A polyatomic interference at m/z 234.8 was successfully removed from the 235U signal under either DRC operating conditions (with or without oxygen as a reaction gas). The method was validated with 15 urine samples of known uranium compositions. The method detection limit for quantification was determined to be 0.1 pg U mL-1 urine and an average coefficient of variation (CV) of 1-2% within the sample measurements. The method detection limit for determining 235U/238U ratio was 3.0 pg U mL-1 urine. An additional 21 patient samples were analyzed with no information about medical history. The measured 235U/238U ratio within the urine samples correctly identified the presence or absence of internal DU exposure in all 21 patients.

Original languageEnglish (US)
Pages (from-to)73-79
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume382
Issue number1
DOIs
StatePublished - May 2005
Externally publishedYes

Keywords

  • Depleted uranium
  • DRC
  • ICP-MS
  • Isotopes
  • Urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Uranium analysis in urine by inductively coupled plasma dynamic reaction cell mass spectrometry'. Together they form a unique fingerprint.

  • Cite this

    Ejnik, J. W., Todorov, T. I., Mullick, F. G., Squibb, K., McDiarmid, M. A., & Centeno, J. A. (2005). Uranium analysis in urine by inductively coupled plasma dynamic reaction cell mass spectrometry. Analytical and Bioanalytical Chemistry, 382(1), 73-79. https://doi.org/10.1007/s00216-005-3173-9