Uptake of [3H]serotonin and [3H]glutamate by primary astrocyte cultures. I. Effects of different sera and time in culture

H. K. Kimelberg, S. K. Goderie, P. A. Conley, Susan Higman, R. Goldschmidt, R. H. Amundson

Research output: Contribution to journalArticle

Abstract

Na+‐dependent, fluoxetine‐sensitive high‐affinity uptake of serotonin and Na+‐dependent uptake of glutamate were studied in primary astrocyte cultures from 1‐day‐old rat neocortex. This uptake was independent of time in culture from 1 to 6 weeks. High‐affinity serotonin uptake was decreased when cells were grown in horse serum as compared to fetal bovine serum and was almost absent when cells were grown in chemically defined medium. In contrast, glutamate uptake was unaffected by the composition of the medium in which the cultures were grown. The serum effect on serotonin uptake was not due to the greater level of serotonin in the fetal bovine serum and was only reversed by a change of serum over a time period of days. © 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalGLIA
Volume6
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Astrocytes
Glutamic Acid
Serotonin
Serum
Neocortex
Horses

Keywords

  • Fluoxetine
  • Serum sensitivity

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Kimelberg, H. K., Goderie, S. K., Conley, P. A., Higman, S., Goldschmidt, R., & Amundson, R. H. (1992). Uptake of [3H]serotonin and [3H]glutamate by primary astrocyte cultures. I. Effects of different sera and time in culture. GLIA, 6(1), 1-8. https://doi.org/10.1002/glia.440060102

Uptake of [3H]serotonin and [3H]glutamate by primary astrocyte cultures. I. Effects of different sera and time in culture. / Kimelberg, H. K.; Goderie, S. K.; Conley, P. A.; Higman, Susan; Goldschmidt, R.; Amundson, R. H.

In: GLIA, Vol. 6, No. 1, 1992, p. 1-8.

Research output: Contribution to journalArticle

Kimelberg, HK, Goderie, SK, Conley, PA, Higman, S, Goldschmidt, R & Amundson, RH 1992, 'Uptake of [3H]serotonin and [3H]glutamate by primary astrocyte cultures. I. Effects of different sera and time in culture', GLIA, vol. 6, no. 1, pp. 1-8. https://doi.org/10.1002/glia.440060102
Kimelberg, H. K. ; Goderie, S. K. ; Conley, P. A. ; Higman, Susan ; Goldschmidt, R. ; Amundson, R. H. / Uptake of [3H]serotonin and [3H]glutamate by primary astrocyte cultures. I. Effects of different sera and time in culture. In: GLIA. 1992 ; Vol. 6, No. 1. pp. 1-8.
@article{f01100339ee447dc81940ffc1a6d0c00,
title = "Uptake of [3H]serotonin and [3H]glutamate by primary astrocyte cultures. I. Effects of different sera and time in culture",
abstract = "Na+‐dependent, fluoxetine‐sensitive high‐affinity uptake of serotonin and Na+‐dependent uptake of glutamate were studied in primary astrocyte cultures from 1‐day‐old rat neocortex. This uptake was independent of time in culture from 1 to 6 weeks. High‐affinity serotonin uptake was decreased when cells were grown in horse serum as compared to fetal bovine serum and was almost absent when cells were grown in chemically defined medium. In contrast, glutamate uptake was unaffected by the composition of the medium in which the cultures were grown. The serum effect on serotonin uptake was not due to the greater level of serotonin in the fetal bovine serum and was only reversed by a change of serum over a time period of days. {\circledC} 1992 Wiley‐Liss, Inc.",
keywords = "Fluoxetine, Serum sensitivity",
author = "Kimelberg, {H. K.} and Goderie, {S. K.} and Conley, {P. A.} and Susan Higman and R. Goldschmidt and Amundson, {R. H.}",
year = "1992",
doi = "10.1002/glia.440060102",
language = "English (US)",
volume = "6",
pages = "1--8",
journal = "GLIA",
issn = "0894-1491",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Uptake of [3H]serotonin and [3H]glutamate by primary astrocyte cultures. I. Effects of different sera and time in culture

AU - Kimelberg, H. K.

AU - Goderie, S. K.

AU - Conley, P. A.

AU - Higman, Susan

AU - Goldschmidt, R.

AU - Amundson, R. H.

PY - 1992

Y1 - 1992

N2 - Na+‐dependent, fluoxetine‐sensitive high‐affinity uptake of serotonin and Na+‐dependent uptake of glutamate were studied in primary astrocyte cultures from 1‐day‐old rat neocortex. This uptake was independent of time in culture from 1 to 6 weeks. High‐affinity serotonin uptake was decreased when cells were grown in horse serum as compared to fetal bovine serum and was almost absent when cells were grown in chemically defined medium. In contrast, glutamate uptake was unaffected by the composition of the medium in which the cultures were grown. The serum effect on serotonin uptake was not due to the greater level of serotonin in the fetal bovine serum and was only reversed by a change of serum over a time period of days. © 1992 Wiley‐Liss, Inc.

AB - Na+‐dependent, fluoxetine‐sensitive high‐affinity uptake of serotonin and Na+‐dependent uptake of glutamate were studied in primary astrocyte cultures from 1‐day‐old rat neocortex. This uptake was independent of time in culture from 1 to 6 weeks. High‐affinity serotonin uptake was decreased when cells were grown in horse serum as compared to fetal bovine serum and was almost absent when cells were grown in chemically defined medium. In contrast, glutamate uptake was unaffected by the composition of the medium in which the cultures were grown. The serum effect on serotonin uptake was not due to the greater level of serotonin in the fetal bovine serum and was only reversed by a change of serum over a time period of days. © 1992 Wiley‐Liss, Inc.

KW - Fluoxetine

KW - Serum sensitivity

UR - http://www.scopus.com/inward/record.url?scp=0026481626&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026481626&partnerID=8YFLogxK

U2 - 10.1002/glia.440060102

DO - 10.1002/glia.440060102

M3 - Article

C2 - 1355074

AN - SCOPUS:0026481626

VL - 6

SP - 1

EP - 8

JO - GLIA

JF - GLIA

SN - 0894-1491

IS - 1

ER -