Uptake of 35S-sulfate by morphologically differentiated replicating chondrocytes in Vivo: A double isotope electron microscope autoradiographic study

Frances A. McHenry; Paul N. Hoffman; Miriam M. Salpeter

doi:10.1016/S0012-1606(74)80011-4

Uptake of ³⁵S-sulfate by morphologically differentiated replicating chondrocytes in Vivo: A double isotope electron microscope autoradiographic study

Frances A. McHenry, Paul N. Hoffman, Miriam M. Salpeter

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Developing cartilage in the 27-day regenerating forelimb of the newt Triturus viridescens was labeled with both ³H-thymidine and ³⁵S-sulfate. The nuclear and cytoplasmic label was assessed quantitatively by EM autoradiography. It was found that chondrocytes incorporated ³H-thymidine into their nuclei while simultaneously incorporating ³⁵S-sulfate in their cytoplasm. The incorporation of ³⁵S-sulfate occurred primarily in the Golgi complex of morphologically differentiated chondrocytes at the base of the developing cartilage, and the ³⁵S uptake by the DNA replicating chondrocytes was quantitatively equivalent per unit area of Golgi to that by the nonreplicating cells.

Original language	English (US)
Pages (from-to)	96-104
Number of pages	9
Journal	Developmental biology
Volume	39
Issue number	1
DOIs	https://doi.org/10.1016/S0012-1606(74)80011-4
State	Published - Jul 1974
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/S0012-1606(74)80011-4

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title = "Uptake of 35S-sulfate by morphologically differentiated replicating chondrocytes in Vivo: A double isotope electron microscope autoradiographic study",

abstract = "Developing cartilage in the 27-day regenerating forelimb of the newt Triturus viridescens was labeled with both 3H-thymidine and 35S-sulfate. The nuclear and cytoplasmic label was assessed quantitatively by EM autoradiography. It was found that chondrocytes incorporated 3H-thymidine into their nuclei while simultaneously incorporating 35S-sulfate in their cytoplasm. The incorporation of 35S-sulfate occurred primarily in the Golgi complex of morphologically differentiated chondrocytes at the base of the developing cartilage, and the 35S uptake by the DNA replicating chondrocytes was quantitatively equivalent per unit area of Golgi to that by the nonreplicating cells.",

author = "McHenry, {Frances A.} and Hoffman, {Paul N.} and Salpeter, {Miriam M.}",

note = "Funding Information: Supported by NIH Grant No. GM10422 from the National Institute of General Medical Sciences. 2 Present address: Department of Anatomy, Western Reserve Medical School, Cleveland, Ohio.",

year = "1974",

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T1 - Uptake of 35S-sulfate by morphologically differentiated replicating chondrocytes in Vivo

T2 - A double isotope electron microscope autoradiographic study

AU - McHenry, Frances A.

AU - Hoffman, Paul N.

AU - Salpeter, Miriam M.

N1 - Funding Information: Supported by NIH Grant No. GM10422 from the National Institute of General Medical Sciences. 2 Present address: Department of Anatomy, Western Reserve Medical School, Cleveland, Ohio.

PY - 1974/7

Y1 - 1974/7

N2 - Developing cartilage in the 27-day regenerating forelimb of the newt Triturus viridescens was labeled with both 3H-thymidine and 35S-sulfate. The nuclear and cytoplasmic label was assessed quantitatively by EM autoradiography. It was found that chondrocytes incorporated 3H-thymidine into their nuclei while simultaneously incorporating 35S-sulfate in their cytoplasm. The incorporation of 35S-sulfate occurred primarily in the Golgi complex of morphologically differentiated chondrocytes at the base of the developing cartilage, and the 35S uptake by the DNA replicating chondrocytes was quantitatively equivalent per unit area of Golgi to that by the nonreplicating cells.

AB - Developing cartilage in the 27-day regenerating forelimb of the newt Triturus viridescens was labeled with both 3H-thymidine and 35S-sulfate. The nuclear and cytoplasmic label was assessed quantitatively by EM autoradiography. It was found that chondrocytes incorporated 3H-thymidine into their nuclei while simultaneously incorporating 35S-sulfate in their cytoplasm. The incorporation of 35S-sulfate occurred primarily in the Golgi complex of morphologically differentiated chondrocytes at the base of the developing cartilage, and the 35S uptake by the DNA replicating chondrocytes was quantitatively equivalent per unit area of Golgi to that by the nonreplicating cells.

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Uptake of ³⁵S-sulfate by morphologically differentiated replicating chondrocytes in Vivo: A double isotope electron microscope autoradiographic study

Abstract

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