Uptake of positron emission tomography tracers in experimental bacterial infections: A comparative biodistribution study of radiolabeled FDG, thymidine, L-methionine, 67Ga-citrate, and 125I-HSA

Yoshifumi Sugawara, Tomasz D. Gutowski, Susan J. Fisher, Raya S. Brown, Richard L. Wahl

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this study was to evaluate the localization of positron emission tomography (PET) tracers [2-deoxy-2-fluoro-D-glucose (FDG), thymidine, and L-methionine] in sites of bacterial infection, and to contrast this with that of other tracers. The left calf muscles of rats were infected with a suspension of Escherichia coli and the biodistribution of 18F- or -3H-FDG, 3H-thymidine, L-11C- or 3H-methionine, gallium-67 citrate (67Ga-citrate) and iodine-125 human serum albumin (125I-HSA) was determined in these animals. 3H-FDG uptake in the infectious foci was evaluated by autoradiography of histological sections. Although 18F-FDG, 67Ga-citrate, and 125I-HSA showed comparatively high uptake in the infected muscle [the percentage activity of injected dose (ID) per gram of tissue normalized for rat weight in kilogram (%ID/g) x kg at 2 h postinjection was as follows: 18F-FDG, 0.184 ± 0.026 to 0.218 ± 0.046; 67Ga-citrate, 0.221 ± 0.016; 125I-HSA, 0.198 ± 0.019], the infected muscle to blood ratio was much higher for 18F-FDG than for 67Ga-citrate or 125I-HSA (18F-FDG, 10.31 ± 0.76 to 14.89 ± 2.26; 67Ga-citrate, 1.24 ± 0.67; 125I-HSA, 0.20 ± 0.02). The draining reactive lymph nodes also showed higher accumulation of 18F-FDG than of 67Ga-citrate or 125I-HSA. The uptake of 3H-thymidine and L-11C- or 3H-methionine in the infected muscle was lower than that of 18F- or 3H-FDG (at 2 h postinjection, 3H-thymidine = 0.039 ± 0.005 and L-3H-methionine = 0.063 ± 0.007 (%ID/g) x kg. Autoradiographs showed that the highest 3H-FDG uptake was seen in the area of inflammatory cell infiltration surrounding the necrotic region. In conclusion, 18F-FDG, which rapidly accumulates in sites of bacterial infection and in reactive lymph nodes with a high target to background ratio, appears to be a promising infection detection agent.

Original languageEnglish (US)
Pages (from-to)333-341
Number of pages9
JournalEuropean Journal of Nuclear Medicine
Volume26
Issue number4
DOIs
StatePublished - 1999

Keywords

  • Bacterial infection
  • Fluorodeoxyglucose thymidine
  • Gallium-67 citrate
  • L-Methionine

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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