TY - JOUR
T1 - Uptake of diflubenzuron (N-[[(4-chlorophenyl)amino] carbonyl]-2,6-difluorobenzamide) by rat C6 glial cells in vitro
AU - Bishai, William R.
AU - Stoolmiller, Allen C.
N1 - Funding Information:
The skillful technical assistance of Helen 0. Hincman and Thomas Savage is gratefully acknowledged. We wish to thank Dr. C. Duane Ferrell for comments on the manuscript. This work was supported by USPHS Grants HD-05515 and HD-04147 and by USDA Cooperative Agreement No. 12-14-5001-269.
PY - 1979/7
Y1 - 1979/7
N2 - The uptake, subcellular localization, and metabolic fate of [14C]diflubenzuron, N-[[(4-chlorophenyl)amino]carbonyl]-2,6-difluorobenzamide, in cultured rat C6 glial cells have been characterized. Confluent monolayer cultures display a concentration-dependent uptake of the labeled insecticide. Experiments conducted in the presence and absence of dimethyl sulfoxide (DMSO) showed that 1.2% DMSO in the growth medium had no significant effect on the rate of incorporation of radioactivity. Diflubenzuron was not metabolized to any measurable extent and more than 98% of the administered compound could be recovered from whole cells and the culture medium by extraction with diethyl ether. As much as 95% of the cell-associated radioactivity was localized in the particulate fraction. Pulse-chase experiments revealed that less than 10% of the [14C]diflubenzuron incorporated during 24 hr of incubation was released into the growth medium over a period of 48 hr. This study in conjunction with our previous investigation (A. C. Stoolmiller, Gen. Pharmacol. 9, 11, 1978) indicates that diflubenzuron is neither cytotoxic nor does it inhibit the synthesis of complex carbohydrates in animal cells.
AB - The uptake, subcellular localization, and metabolic fate of [14C]diflubenzuron, N-[[(4-chlorophenyl)amino]carbonyl]-2,6-difluorobenzamide, in cultured rat C6 glial cells have been characterized. Confluent monolayer cultures display a concentration-dependent uptake of the labeled insecticide. Experiments conducted in the presence and absence of dimethyl sulfoxide (DMSO) showed that 1.2% DMSO in the growth medium had no significant effect on the rate of incorporation of radioactivity. Diflubenzuron was not metabolized to any measurable extent and more than 98% of the administered compound could be recovered from whole cells and the culture medium by extraction with diethyl ether. As much as 95% of the cell-associated radioactivity was localized in the particulate fraction. Pulse-chase experiments revealed that less than 10% of the [14C]diflubenzuron incorporated during 24 hr of incubation was released into the growth medium over a period of 48 hr. This study in conjunction with our previous investigation (A. C. Stoolmiller, Gen. Pharmacol. 9, 11, 1978) indicates that diflubenzuron is neither cytotoxic nor does it inhibit the synthesis of complex carbohydrates in animal cells.
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U2 - 10.1016/0048-3575(79)90065-8
DO - 10.1016/0048-3575(79)90065-8
M3 - Article
AN - SCOPUS:0018751579
VL - 11
SP - 258
EP - 266
JO - Pesticide Biochemistry and Physiology
JF - Pesticide Biochemistry and Physiology
SN - 0048-3575
IS - 1-3
ER -