Uptake and transport of superparamagnetic iron oxide nanoparticles through human brain capillary endothelial cells

L. B. Thomsen, T. Linemann, K. M. Pondman, J. Lichota, Kwang Sik Kim, R. J. Pieters, G. M. Visser, T. Moos

Research output: Contribution to journalArticle

Abstract

The blood-brain barrier (BBB) formed by brain capillary endothelial cells (BCECs) constitutes a firm physical, chemical, and immunological barrier, making the brain accessible to only a few percent of potential drugs intended for treatment inside the central nervous system. With the purpose of overcoming the restraints of the BBB by allowing the transport of drugs, siRNA, or DNA into the brain, a novel approach is to use superparamagnetic iron oxide nanoparticles (SPIONs) as drug carriers. The aim of this study was to investigate the ability of fluorescent SPIONs to pass through human brain microvascular endothelial cells facilitated by an external magnet. The ability of SPIONs to penetrate the barrier was shown to be significantly stronger in the presence of an external magnetic force in an in vitro BBB model. Hence, particles added to the luminal side of the in vitro BBB model were found in astrocytes cocultured at a remote distance on the abluminal side, indicating that particles were transported through the barrier and taken up by astrocytes. Addition of the SPIONs to the culture medium did not negatively affect the viability of the endothelial cells. The magnetic force-mediated dragging of SPIONs through BCECs may denote a novel mechanism for the delivery of drugs to the brain.

Original languageEnglish (US)
Pages (from-to)1352-1360
Number of pages9
JournalACS Chemical Neuroscience
Volume4
Issue number10
DOIs
StatePublished - Oct 16 2013

Fingerprint

Endothelial cells
Nanoparticles
Brain
Endothelial Cells
Blood-Brain Barrier
Astrocytes
Pharmaceutical Preparations
Drug Carriers
Magnets
Neurology
Small Interfering RNA
Culture Media
ferric oxide
Central Nervous System
DNA

Keywords

  • astrocytes
  • blood-brain barrier
  • drug delivery
  • endothelial cells
  • in vitro
  • magnetic nanoparticles
  • TEER

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Cognitive Neuroscience

Cite this

Uptake and transport of superparamagnetic iron oxide nanoparticles through human brain capillary endothelial cells. / Thomsen, L. B.; Linemann, T.; Pondman, K. M.; Lichota, J.; Kim, Kwang Sik; Pieters, R. J.; Visser, G. M.; Moos, T.

In: ACS Chemical Neuroscience, Vol. 4, No. 10, 16.10.2013, p. 1352-1360.

Research output: Contribution to journalArticle

Thomsen, LB, Linemann, T, Pondman, KM, Lichota, J, Kim, KS, Pieters, RJ, Visser, GM & Moos, T 2013, 'Uptake and transport of superparamagnetic iron oxide nanoparticles through human brain capillary endothelial cells', ACS Chemical Neuroscience, vol. 4, no. 10, pp. 1352-1360. https://doi.org/10.1021/cn400093z
Thomsen, L. B. ; Linemann, T. ; Pondman, K. M. ; Lichota, J. ; Kim, Kwang Sik ; Pieters, R. J. ; Visser, G. M. ; Moos, T. / Uptake and transport of superparamagnetic iron oxide nanoparticles through human brain capillary endothelial cells. In: ACS Chemical Neuroscience. 2013 ; Vol. 4, No. 10. pp. 1352-1360.
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