Ups1p, a conserved intermembrane space protein, regulates mitochondrial shape and alternative topogenesis of Mgm1p

Hiromi Sesaki, Cory D. Dunn, Miho Iijima, Kelly A. Shepard, Michael P. Yaffe, Carolyn E Machamer, Robert E. Jensen

Research output: Contribution to journalArticle

Abstract

Mgm1p is a conserved dynamin-related GTPase required for fusion, morphology, inheritance, and the genome maintenance of mitochondria in Saccharomyces cerevisiae. Mgm1p undergoes unconventional processing to produce two functional isoforms by alternative topogenesis. Alternative topogenesis involves bifurcate sorting in the inner membrane and intramembrane proteolysis by the rhomboid protease Pcp1p. Here, we identify Ups1p, a novel mitochondrial protein required for the unique processing of Mgm1p and for normal mitochondrial shape. Our results demonstrate that Ups1p regulates the sorting of Mgm1p in the inner membrane. Consistent with its function, Ups1p is peripherally associated with the inner membrane in the intermembrane space. Moreover, the human homologue of Ups1p, PRELI, can fully replace Ups1p in yeast cells. Together, our findings provide a conserved mechanism for the alternative topogenesis of Mgm1p and control of mitochondrial morphology.

Original languageEnglish (US)
Pages (from-to)651-658
Number of pages8
JournalJournal of Cell Biology
Volume173
Issue number5
DOIs
StatePublished - Jun 5 2006

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Mitochondrial Proteins
Membranes
Dynamins
GTP Phosphohydrolases
Proteolysis
Saccharomyces cerevisiae
Mitochondria
Protein Isoforms
Peptide Hydrolases
Yeasts
Maintenance
Genome

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ups1p, a conserved intermembrane space protein, regulates mitochondrial shape and alternative topogenesis of Mgm1p. / Sesaki, Hiromi; Dunn, Cory D.; Iijima, Miho; Shepard, Kelly A.; Yaffe, Michael P.; Machamer, Carolyn E; Jensen, Robert E.

In: Journal of Cell Biology, Vol. 173, No. 5, 05.06.2006, p. 651-658.

Research output: Contribution to journalArticle

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AU - Sesaki, Hiromi

AU - Dunn, Cory D.

AU - Iijima, Miho

AU - Shepard, Kelly A.

AU - Yaffe, Michael P.

AU - Machamer, Carolyn E

AU - Jensen, Robert E.

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AB - Mgm1p is a conserved dynamin-related GTPase required for fusion, morphology, inheritance, and the genome maintenance of mitochondria in Saccharomyces cerevisiae. Mgm1p undergoes unconventional processing to produce two functional isoforms by alternative topogenesis. Alternative topogenesis involves bifurcate sorting in the inner membrane and intramembrane proteolysis by the rhomboid protease Pcp1p. Here, we identify Ups1p, a novel mitochondrial protein required for the unique processing of Mgm1p and for normal mitochondrial shape. Our results demonstrate that Ups1p regulates the sorting of Mgm1p in the inner membrane. Consistent with its function, Ups1p is peripherally associated with the inner membrane in the intermembrane space. Moreover, the human homologue of Ups1p, PRELI, can fully replace Ups1p in yeast cells. Together, our findings provide a conserved mechanism for the alternative topogenesis of Mgm1p and control of mitochondrial morphology.

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