Upregulation of nitric oxide synthase and galanin message-associated peptide in hypothalamic magnocellular neurons after hypophysectomy. Immunohistochemical and in situ hybridization studies

Marcelo J. Villar, Sandra Ceccatelli, Katarina Bedecs, Tamas Bartfai, David Bredt, Solomon H Snyder, Tomas Hökfelt

Research output: Contribution to journalArticle

Abstract

The expression of several bioactive molecules in magnocellular hypothalamic neurons is modified when the axons of these cells are transected. In this stuyd we have evaluated by means of immunocytochemistry and in situ by hybridization the effect of hypophysectomy on the expression of nitric oxide synthase (NOS)- and of galanin message-associated peptide (GMAP)-like immunoreactivities (-LIs) as well as on their respective mRNAs in hypothalamic magnocellular neurosecretory neurons. The results show a transient increase in NOS- and GMAP-LIs in magnocellular neurons of both the paraventricular and supraoptic nuclei when compared to normal animals. The maximal increase in staining was observed between 5 and 7 days, and by 14 days NOS-LI was back to normal levels, whereas strong GMP-LI could still be detected in a few cella. A similar picture was observed for the NOS and GMAP mRNAs. The functional significance of the present findings is unclear, but they indicate a possible role of nitric oxide and GMAP in neurosecretory neurons after injury.

Original languageEnglish (US)
Pages (from-to)219-228
Number of pages10
JournalBrain Research
Volume650
Issue number2
DOIs
StatePublished - Jul 11 1994

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Hypophysectomy
Nitric Oxide Synthase
In Situ Hybridization
Up-Regulation
Neurons
Supraoptic Nucleus
Messenger RNA
Paraventricular Hypothalamic Nucleus
Axons
Nitric Oxide
Immunohistochemistry
Staining and Labeling
galanin message-associated peptide
Wounds and Injuries

Keywords

  • Injury
  • Nitric oxide
  • Paraventricular nucleus
  • Regeneration
  • Supraoptic nucleus

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Upregulation of nitric oxide synthase and galanin message-associated peptide in hypothalamic magnocellular neurons after hypophysectomy. Immunohistochemical and in situ hybridization studies. / Villar, Marcelo J.; Ceccatelli, Sandra; Bedecs, Katarina; Bartfai, Tamas; Bredt, David; Snyder, Solomon H; Hökfelt, Tomas.

In: Brain Research, Vol. 650, No. 2, 11.07.1994, p. 219-228.

Research output: Contribution to journalArticle

Villar, Marcelo J. ; Ceccatelli, Sandra ; Bedecs, Katarina ; Bartfai, Tamas ; Bredt, David ; Snyder, Solomon H ; Hökfelt, Tomas. / Upregulation of nitric oxide synthase and galanin message-associated peptide in hypothalamic magnocellular neurons after hypophysectomy. Immunohistochemical and in situ hybridization studies. In: Brain Research. 1994 ; Vol. 650, No. 2. pp. 219-228.
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AU - Hökfelt, Tomas

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