Up-regulation of natriuretic peptides in the ventricle of Csx/Nkx2-5 transgenic mice

Eiki Takimoto; Takehiko Mizuno; Fumio Terasaki; Masaki Shimoyama; Hiroaki Honda; Ichiro Shiojima; Yukio Hiroi; Toru Oka; Doubun Hayashi; Hisamaru Hirai; Sumiyo Kudoh; Haruhiko Toko; Keishiro Kawamura; Ryozo Nagai; Yoshio Yazaki; Issei Komuro

doi:10.1006/bbrc.2000.2561

Up-regulation of natriuretic peptides in the ventricle of Csx/Nkx2-5 transgenic mice

Eiki Takimoto, Takehiko Mizuno, Fumio Terasaki, Masaki Shimoyama, Hiroaki Honda, Ichiro Shiojima, Yukio Hiroi, Toru Oka, Doubun Hayashi, Hisamaru Hirai, Sumiyo Kudoh, Haruhiko Toko, Keishiro Kawamura, Ryozo Nagai, Yoshio Yazaki, Issei Komuro

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29 Scopus citations

Abstract

A cardiac homeobox-containing gene Csx/NKx2-5, which is essential for cardiac development, is abundantly expressed in the adult heart as well as in the heart primordia. Targeted disruption of this gene results in embryonic lethality due to abnormal heart morphogenesis. To elucidate the role of Csx/Nkx2-5 in the adult heart, we generated transgenic mice which overexpress human Csx/Nkx2-5. The transgene was expressed abundantly in the heart and the skeletal muscle. mRNA levels of several cardiac genes including natriuretic peptides, CARP, MLC2v, and endogenous Csx/Nkx2-5 were increased in the ventricle of the transgenic mice. Electron microscopic analysis revealed that the ventricular myocardium of the transgenic mice had many secretory granules, which disappeared after administration of vasopressin. These results suggest that Csx/Nkx2-5 regulates many cardiac genes and induces formation of secretory granules in the adult ventricle. (C) 2000 Academic Press.

Original language	English (US)
Pages (from-to)	1074-1079
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	270
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.2000.2561
State	Published - Apr 21 2000
Externally published	Yes

Keywords

Atrial natriuretic peptide
Csx/Nkx2-5
Electron microscopic analysis
Secretory granules
Transgenic mice

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1006/bbrc.2000.2561

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Takimoto, E., Mizuno, T., Terasaki, F., Shimoyama, M., Honda, H., Shiojima, I., Hiroi, Y., Oka, T., Hayashi, D., Hirai, H., Kudoh, S., Toko, H., Kawamura, K., Nagai, R., Yazaki, Y., & Komuro, I. (2000). Up-regulation of natriuretic peptides in the ventricle of Csx/Nkx2-5 transgenic mice. Biochemical and Biophysical Research Communications, 270(3), 1074-1079. https://doi.org/10.1006/bbrc.2000.2561

Takimoto, E, Mizuno, T, Terasaki, F, Shimoyama, M, Honda, H, Shiojima, I, Hiroi, Y, Oka, T, Hayashi, D, Hirai, H, Kudoh, S, Toko, H, Kawamura, K, Nagai, R, Yazaki, Y & Komuro, I 2000, 'Up-regulation of natriuretic peptides in the ventricle of Csx/Nkx2-5 transgenic mice', Biochemical and Biophysical Research Communications, vol. 270, no. 3, pp. 1074-1079. https://doi.org/10.1006/bbrc.2000.2561

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abstract = "A cardiac homeobox-containing gene Csx/NKx2-5, which is essential for cardiac development, is abundantly expressed in the adult heart as well as in the heart primordia. Targeted disruption of this gene results in embryonic lethality due to abnormal heart morphogenesis. To elucidate the role of Csx/Nkx2-5 in the adult heart, we generated transgenic mice which overexpress human Csx/Nkx2-5. The transgene was expressed abundantly in the heart and the skeletal muscle. mRNA levels of several cardiac genes including natriuretic peptides, CARP, MLC2v, and endogenous Csx/Nkx2-5 were increased in the ventricle of the transgenic mice. Electron microscopic analysis revealed that the ventricular myocardium of the transgenic mice had many secretory granules, which disappeared after administration of vasopressin. These results suggest that Csx/Nkx2-5 regulates many cardiac genes and induces formation of secretory granules in the adult ventricle. (C) 2000 Academic Press.",

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AU - Takimoto, Eiki

AU - Mizuno, Takehiko

AU - Terasaki, Fumio

AU - Shimoyama, Masaki

AU - Honda, Hiroaki

AU - Shiojima, Ichiro

AU - Hiroi, Yukio

AU - Oka, Toru

AU - Hayashi, Doubun

AU - Hirai, Hisamaru

AU - Kudoh, Sumiyo

AU - Toko, Haruhiko

AU - Kawamura, Keishiro

AU - Nagai, Ryozo

AU - Yazaki, Yoshio

AU - Komuro, Issei

PY - 2000/4/21

Y1 - 2000/4/21

N2 - A cardiac homeobox-containing gene Csx/NKx2-5, which is essential for cardiac development, is abundantly expressed in the adult heart as well as in the heart primordia. Targeted disruption of this gene results in embryonic lethality due to abnormal heart morphogenesis. To elucidate the role of Csx/Nkx2-5 in the adult heart, we generated transgenic mice which overexpress human Csx/Nkx2-5. The transgene was expressed abundantly in the heart and the skeletal muscle. mRNA levels of several cardiac genes including natriuretic peptides, CARP, MLC2v, and endogenous Csx/Nkx2-5 were increased in the ventricle of the transgenic mice. Electron microscopic analysis revealed that the ventricular myocardium of the transgenic mice had many secretory granules, which disappeared after administration of vasopressin. These results suggest that Csx/Nkx2-5 regulates many cardiac genes and induces formation of secretory granules in the adult ventricle. (C) 2000 Academic Press.

AB - A cardiac homeobox-containing gene Csx/NKx2-5, which is essential for cardiac development, is abundantly expressed in the adult heart as well as in the heart primordia. Targeted disruption of this gene results in embryonic lethality due to abnormal heart morphogenesis. To elucidate the role of Csx/Nkx2-5 in the adult heart, we generated transgenic mice which overexpress human Csx/Nkx2-5. The transgene was expressed abundantly in the heart and the skeletal muscle. mRNA levels of several cardiac genes including natriuretic peptides, CARP, MLC2v, and endogenous Csx/Nkx2-5 were increased in the ventricle of the transgenic mice. Electron microscopic analysis revealed that the ventricular myocardium of the transgenic mice had many secretory granules, which disappeared after administration of vasopressin. These results suggest that Csx/Nkx2-5 regulates many cardiac genes and induces formation of secretory granules in the adult ventricle. (C) 2000 Academic Press.

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Up-regulation of natriuretic peptides in the ventricle of Csx/Nkx2-5 transgenic mice

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