Up-regulation of natriuretic peptides in the ventricle of Csx/Nkx2-5 transgenic mice

Eiki Takimoto, Takehiko Mizuno, Fumio Terasaki, Masaki Shimoyama, Hiroaki Honda, Ichiro Shiojima, Yukio Hiroi, Toru Oka, Doubun Hayashi, Hisamaru Hirai, Sumiyo Kudoh, Haruhiko Toko, Keishiro Kawamura, Ryozo Nagai, Yoshio Yazaki, Issei Komuro

Research output: Contribution to journalArticlepeer-review

Abstract

A cardiac homeobox-containing gene Csx/NKx2-5, which is essential for cardiac development, is abundantly expressed in the adult heart as well as in the heart primordia. Targeted disruption of this gene results in embryonic lethality due to abnormal heart morphogenesis. To elucidate the role of Csx/Nkx2-5 in the adult heart, we generated transgenic mice which overexpress human Csx/Nkx2-5. The transgene was expressed abundantly in the heart and the skeletal muscle. mRNA levels of several cardiac genes including natriuretic peptides, CARP, MLC2v, and endogenous Csx/Nkx2-5 were increased in the ventricle of the transgenic mice. Electron microscopic analysis revealed that the ventricular myocardium of the transgenic mice had many secretory granules, which disappeared after administration of vasopressin. These results suggest that Csx/Nkx2-5 regulates many cardiac genes and induces formation of secretory granules in the adult ventricle. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)1074-1079
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume270
Issue number3
DOIs
StatePublished - Apr 21 2000
Externally publishedYes

Keywords

  • Atrial natriuretic peptide
  • Csx/Nkx2-5
  • Electron microscopic analysis
  • Secretory granules
  • Transgenic mice

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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