Up-regulation of endothelial nitric oxide synthase expression by cyclic guanosine 3′,5′-monophosphate

Lingamanaidu V. Ravichandran, Roger A Johns

Research output: Contribution to journalArticle

Abstract

In this study, the role of cyclic GMP, the end product of the NO-cyclic GMP signalling pathway, in the regulation of ecNOS was investigated. Bovine pulmonary endothelial cells were exposed to 8-bromo-cyclic GMP and its effect on NO production, ecNOS protein, and mRNA levels was analyzed. Endothelial cells on exposure to 8-bromo-cyclic GMP produced significantly increased amounts of NO, detected as increased cyclic GMP in cocultures with vascular smooth muscle cells both under basal conditions and with agonist stimulation. 8-Bromo-cyclic GMP significantly increased the ecNOS protein and mRNA levels as detected on Western and Northern blots respectively. This 8-bromo-cyclic GMP mediated increase of NO production, ecNOS protein and mRNA levels suggests that cyclic GMP up-regulates the expression of ecNOS. Thus, there may be an intercellular feedback mechanism involved at the molecular level in the expression of the NO-cyclic GMP signalling pathway in blood vessels.

Original languageEnglish (US)
Pages (from-to)295-298
Number of pages4
JournalFEBS Letters
Volume374
Issue number2
DOIs
StatePublished - Oct 30 1995
Externally publishedYes

Fingerprint

Guanosine Monophosphate
Nitric Oxide Synthase Type III
Cyclic GMP
Up-Regulation
Endothelial cells
Messenger RNA
Endothelial Cells
Proteins
Blood vessels
Coculture Techniques
Vascular Smooth Muscle
Northern Blotting
Smooth Muscle Myocytes
Blood Vessels
Muscle
Western Blotting
Cells
Feedback
Lung
8-bromocyclic GMP

Keywords

  • Cyclic GMP
  • Molecular expression
  • NO synthase
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Up-regulation of endothelial nitric oxide synthase expression by cyclic guanosine 3′,5′-monophosphate. / Ravichandran, Lingamanaidu V.; Johns, Roger A.

In: FEBS Letters, Vol. 374, No. 2, 30.10.1995, p. 295-298.

Research output: Contribution to journalArticle

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N2 - In this study, the role of cyclic GMP, the end product of the NO-cyclic GMP signalling pathway, in the regulation of ecNOS was investigated. Bovine pulmonary endothelial cells were exposed to 8-bromo-cyclic GMP and its effect on NO production, ecNOS protein, and mRNA levels was analyzed. Endothelial cells on exposure to 8-bromo-cyclic GMP produced significantly increased amounts of NO, detected as increased cyclic GMP in cocultures with vascular smooth muscle cells both under basal conditions and with agonist stimulation. 8-Bromo-cyclic GMP significantly increased the ecNOS protein and mRNA levels as detected on Western and Northern blots respectively. This 8-bromo-cyclic GMP mediated increase of NO production, ecNOS protein and mRNA levels suggests that cyclic GMP up-regulates the expression of ecNOS. Thus, there may be an intercellular feedback mechanism involved at the molecular level in the expression of the NO-cyclic GMP signalling pathway in blood vessels.

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