Up-regulation effect of hydrogen peroxide on expression of 20S proteasome in ARPE-19

Yan Yan, Xia Lei, Ling Wang, Guo Tong Xu

Research output: Contribution to journalArticle

Abstract

• Aim: To investigate the effect of hydrogen peroxide on 20S proteasome expression induced by oxidative stress in ARPE-19 cells in vitro. • Methods: ARPE-19 were treated with 0, 100 or 300μ mol/L hydrogen peroxide for 4 hours, respectively, then the expression and distribution of 20S proteasome in cells were detected by Western Blot and immunofluorescence staining assay respectively. • Results: 20S proteasome increased significantly in ARPE-19 when it treated by hydrogen peroxide at 100 and 300μ mol/L (100μ mol/L hydrogen peroxide vs 0, 300μ mol/L hydrogen peroxide, P < 0.05), and the up-regulation change was dose-dependent. Intracellular distribution of 20S proteasome in ARPE-19 cultured under normal circumstances was around the nuclear membrane. 20S proteasome expression in ARPE-19 increased after treated by hydrogen peroxide at 100 and 300μ mol/L, mainly in the nucleus and around the nuclear membrane. • Conclusion: 20S proteasome is up-regulated in ARPE-19 treated by hydrogen peroxide. Ubiquitin-proteasome pathway is induced in ARPE-19 by oxidative stress in vitro.

Original languageEnglish (US)
Pages (from-to)1156-1158
Number of pages3
JournalInternational Journal of Ophthalmology
Volume8
Issue number6
StatePublished - Jun 25 2008
Externally publishedYes

Fingerprint

Proteasome Endopeptidase Complex
Hydrogen Peroxide
Up-Regulation
Nuclear Envelope
Oxidative Stress
Ubiquitin
Fluorescent Antibody Technique
Western Blotting
Staining and Labeling

Keywords

  • Hydrogen peroxide
  • Oxidative stress
  • Proteasome
  • Retinal pigment epithelial cell

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Up-regulation effect of hydrogen peroxide on expression of 20S proteasome in ARPE-19. / Yan, Yan; Lei, Xia; Wang, Ling; Xu, Guo Tong.

In: International Journal of Ophthalmology, Vol. 8, No. 6, 25.06.2008, p. 1156-1158.

Research output: Contribution to journalArticle

Yan, Yan ; Lei, Xia ; Wang, Ling ; Xu, Guo Tong. / Up-regulation effect of hydrogen peroxide on expression of 20S proteasome in ARPE-19. In: International Journal of Ophthalmology. 2008 ; Vol. 8, No. 6. pp. 1156-1158.
@article{06177985c1d4456fa03c63c6dd982d20,
title = "Up-regulation effect of hydrogen peroxide on expression of 20S proteasome in ARPE-19",
abstract = "• Aim: To investigate the effect of hydrogen peroxide on 20S proteasome expression induced by oxidative stress in ARPE-19 cells in vitro. • Methods: ARPE-19 were treated with 0, 100 or 300μ mol/L hydrogen peroxide for 4 hours, respectively, then the expression and distribution of 20S proteasome in cells were detected by Western Blot and immunofluorescence staining assay respectively. • Results: 20S proteasome increased significantly in ARPE-19 when it treated by hydrogen peroxide at 100 and 300μ mol/L (100μ mol/L hydrogen peroxide vs 0, 300μ mol/L hydrogen peroxide, P < 0.05), and the up-regulation change was dose-dependent. Intracellular distribution of 20S proteasome in ARPE-19 cultured under normal circumstances was around the nuclear membrane. 20S proteasome expression in ARPE-19 increased after treated by hydrogen peroxide at 100 and 300μ mol/L, mainly in the nucleus and around the nuclear membrane. • Conclusion: 20S proteasome is up-regulated in ARPE-19 treated by hydrogen peroxide. Ubiquitin-proteasome pathway is induced in ARPE-19 by oxidative stress in vitro.",
keywords = "Hydrogen peroxide, Oxidative stress, Proteasome, Retinal pigment epithelial cell",
author = "Yan Yan and Xia Lei and Ling Wang and Xu, {Guo Tong}",
year = "2008",
month = "6",
day = "25",
language = "English (US)",
volume = "8",
pages = "1156--1158",
journal = "International Eye Science",
issn = "2222-3959",
publisher = "Press of International Journal of Ophthalmology",
number = "6",

}

TY - JOUR

T1 - Up-regulation effect of hydrogen peroxide on expression of 20S proteasome in ARPE-19

AU - Yan, Yan

AU - Lei, Xia

AU - Wang, Ling

AU - Xu, Guo Tong

PY - 2008/6/25

Y1 - 2008/6/25

N2 - • Aim: To investigate the effect of hydrogen peroxide on 20S proteasome expression induced by oxidative stress in ARPE-19 cells in vitro. • Methods: ARPE-19 were treated with 0, 100 or 300μ mol/L hydrogen peroxide for 4 hours, respectively, then the expression and distribution of 20S proteasome in cells were detected by Western Blot and immunofluorescence staining assay respectively. • Results: 20S proteasome increased significantly in ARPE-19 when it treated by hydrogen peroxide at 100 and 300μ mol/L (100μ mol/L hydrogen peroxide vs 0, 300μ mol/L hydrogen peroxide, P < 0.05), and the up-regulation change was dose-dependent. Intracellular distribution of 20S proteasome in ARPE-19 cultured under normal circumstances was around the nuclear membrane. 20S proteasome expression in ARPE-19 increased after treated by hydrogen peroxide at 100 and 300μ mol/L, mainly in the nucleus and around the nuclear membrane. • Conclusion: 20S proteasome is up-regulated in ARPE-19 treated by hydrogen peroxide. Ubiquitin-proteasome pathway is induced in ARPE-19 by oxidative stress in vitro.

AB - • Aim: To investigate the effect of hydrogen peroxide on 20S proteasome expression induced by oxidative stress in ARPE-19 cells in vitro. • Methods: ARPE-19 were treated with 0, 100 or 300μ mol/L hydrogen peroxide for 4 hours, respectively, then the expression and distribution of 20S proteasome in cells were detected by Western Blot and immunofluorescence staining assay respectively. • Results: 20S proteasome increased significantly in ARPE-19 when it treated by hydrogen peroxide at 100 and 300μ mol/L (100μ mol/L hydrogen peroxide vs 0, 300μ mol/L hydrogen peroxide, P < 0.05), and the up-regulation change was dose-dependent. Intracellular distribution of 20S proteasome in ARPE-19 cultured under normal circumstances was around the nuclear membrane. 20S proteasome expression in ARPE-19 increased after treated by hydrogen peroxide at 100 and 300μ mol/L, mainly in the nucleus and around the nuclear membrane. • Conclusion: 20S proteasome is up-regulated in ARPE-19 treated by hydrogen peroxide. Ubiquitin-proteasome pathway is induced in ARPE-19 by oxidative stress in vitro.

KW - Hydrogen peroxide

KW - Oxidative stress

KW - Proteasome

KW - Retinal pigment epithelial cell

UR - http://www.scopus.com/inward/record.url?scp=47249154770&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47249154770&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:47249154770

VL - 8

SP - 1156

EP - 1158

JO - International Eye Science

JF - International Eye Science

SN - 2222-3959

IS - 6

ER -