Unsuspected primary human immunodeficiency virus type 1 infection in seronegative emergency department patients

Stephen J. Clark, Gabor D Kelen, Denis R. Henrard, Eric S. Daar, Steven Craig, George M. Shaw, Thomas C Quinn

Research output: Contribution to journalArticle

Abstract

To estimate the number of recently infected patients in the window period before human immunodeficiency virus type 1 (HIV-1) seroconversion among patients seeking medical care, randomly selected adults presenting to an inner city emergency department were tested for HIV1 antibody and p24 antigen. Of 2300 patients enrolled, 180 (7.8%; 95% confidence interval [CI]: 6.7%-8.9%) were Western blot (WB)-positive for HIV-1 antibodies. Of 2120 antibody-negative or WB-indeterminate patients, none of whom were identified on clinical grounds as having primary HIV-1 infection, 6 (0.28%; CI, 0.07%-0.51%) were p24 antigen-positive with serologies consistent with primary HIV-1 infection. Of these 6, 3 were seronegative even with third-generation antibody ELISA. HIV-1 infection in these 6 patients was further confirmed by polymerase chain reaction amplification of virion-associated RNA in serum demonstrating 104-105 virions/mL. With 40, 000 new HIV-1 infections in the United States annually, 750 persons with undiagnosed primary HIV-1 infections may seek primary health care in any given week in the United States. Testing for viral antibodies alone will fail to detect a large proportion of these persons. Thus, early identification by p24 antigen testing may be important to diagnose and treat symptomatic illness, implement public health and counseling measures, and arrange appropriate medical follow-up.

Original languageEnglish (US)
Pages (from-to)194-197
Number of pages4
JournalJournal of Infectious Diseases
Volume170
Issue number1
DOIs
StatePublished - 1994

Fingerprint

Virus Diseases
HIV-1
Hospital Emergency Service
Antibodies
Antigens
Virion
Western Blotting
Viral Antibodies
Confidence Intervals
Serology
Counseling
Primary Health Care
Public Health
Enzyme-Linked Immunosorbent Assay
RNA
Polymerase Chain Reaction
Serum

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Unsuspected primary human immunodeficiency virus type 1 infection in seronegative emergency department patients. / Clark, Stephen J.; Kelen, Gabor D; Henrard, Denis R.; Daar, Eric S.; Craig, Steven; Shaw, George M.; Quinn, Thomas C.

In: Journal of Infectious Diseases, Vol. 170, No. 1, 1994, p. 194-197.

Research output: Contribution to journalArticle

Clark, Stephen J. ; Kelen, Gabor D ; Henrard, Denis R. ; Daar, Eric S. ; Craig, Steven ; Shaw, George M. ; Quinn, Thomas C. / Unsuspected primary human immunodeficiency virus type 1 infection in seronegative emergency department patients. In: Journal of Infectious Diseases. 1994 ; Vol. 170, No. 1. pp. 194-197.
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