Unsupervised segmentation of multiparameter MRI in experimental cerebral ischemia with comparison to T2, diffusion, and ADC MRI parameters and histopathological validation

Michael Jacobs, Robert A. Knight, Hamid Soltanian-Zadeh, Zhang G. Zheng, Anton V. Goussev, Donald J. Peck, Joe P. Windham, Michael Chopp

Research output: Contribution to journalArticle

Abstract

This study presents histological validation of an objective (unsupervised) computer segmentation algorithm, the iterative self-organizing data analysis technique (ISODATA), for analysis of multiparameter magnetic resonance imaging (MRI) data in experimental focal cerebral ischemia. T2-, T1-, and diffusion (DWI) weighted coronal images were acquired from 4 to 168 hours after stroke on separate groups of animals. Animals were killed immediately after MRI for histological analysis. MR images were coregistered/warped to histology. MRI lesion areas were defined using DWI, apparent diffusion coefficient (ADC) maps, T2-weighted images, and ISODATA. The last techniques clearly discriminated between ischemia-altered and morphologically intact tissue. ISODATA areas were congruent and significantly correlated (r = 0.99, P <0.05) with histologically defined lesions. In contrast, DWI, ADC, and T2 lesion areas showed no significant correlation with histologically evaluated lesions until subacute time points. These data indicate that multiparameter ISODATA methodology can accurately detect and identify ischemic cell damage early and late after ischemia, with ISODATA outperforming ADC, DWI, and T2-weighted images in identification of ischemic lesions from 4 to 168 hours after stroke. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)425-437
Number of pages13
JournalJournal of Magnetic Resonance Imaging
Volume11
Issue number4
DOIs
StatePublished - Apr 2000

Fingerprint

Diffusion Magnetic Resonance Imaging
Brain Ischemia
Magnetic Resonance Imaging
Ischemia
Stroke
Histology

Keywords

  • Cerebral ischemia
  • Computer assisted
  • Diffusion
  • Histopathology (rat)
  • Image processing
  • ISODATA
  • Magnetic resonance imaging
  • Tissue signature model

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Unsupervised segmentation of multiparameter MRI in experimental cerebral ischemia with comparison to T2, diffusion, and ADC MRI parameters and histopathological validation. / Jacobs, Michael; Knight, Robert A.; Soltanian-Zadeh, Hamid; Zheng, Zhang G.; Goussev, Anton V.; Peck, Donald J.; Windham, Joe P.; Chopp, Michael.

In: Journal of Magnetic Resonance Imaging, Vol. 11, No. 4, 04.2000, p. 425-437.

Research output: Contribution to journalArticle

Jacobs, Michael ; Knight, Robert A. ; Soltanian-Zadeh, Hamid ; Zheng, Zhang G. ; Goussev, Anton V. ; Peck, Donald J. ; Windham, Joe P. ; Chopp, Michael. / Unsupervised segmentation of multiparameter MRI in experimental cerebral ischemia with comparison to T2, diffusion, and ADC MRI parameters and histopathological validation. In: Journal of Magnetic Resonance Imaging. 2000 ; Vol. 11, No. 4. pp. 425-437.
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