Unscheduled DNA synthesis in cultured ataxia telangiectasia fibroblast-like cells

R. A. Vincent; A. J. Fink; P. C. Huang

doi:10.1016/0027-5107(80)90038-X

Unscheduled DNA synthesis in cultured ataxia telangiectasia fibroblast-like cells

R. A. Vincent, A. J. Fink, P. C. Huang

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The capacity of cultured fibroblast-like cells from 5 ataxia telangiectasia (AT) patients to repair γ-ray-induced damage formed in DNA under aerobic conditions was measured by unscheduled DNA synthesis (UDS). The AT cultures exhibited generally reduced UDS compared to the normal cultures for γ-ray doses of 4–12 kR. Reduced UDS in the AT cultures was observed for repair-incubation periods at which UDS was not saturated (0.5 and 1 h), and for periods characterized by maximal amounts of UDS in both the normal and AT cultures (2 and 4 h). The results suggest a class of lesions repairable in the normal cells by UDS within 1 h many persist in the AT cells for 4 h or longer.

Original language	English (US)
Pages (from-to)	245-249
Number of pages	5
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	72
Issue number	2
DOIs	https://doi.org/10.1016/0027-5107(80)90038-X
State	Published - 1980
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

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@article{c8b4d426bf5e49cf97e61b34adde057c,

title = "Unscheduled DNA synthesis in cultured ataxia telangiectasia fibroblast-like cells",

abstract = "The capacity of cultured fibroblast-like cells from 5 ataxia telangiectasia (AT) patients to repair γ-ray-induced damage formed in DNA under aerobic conditions was measured by unscheduled DNA synthesis (UDS). The AT cultures exhibited generally reduced UDS compared to the normal cultures for γ-ray doses of 4–12 kR. Reduced UDS in the AT cultures was observed for repair-incubation periods at which UDS was not saturated (0.5 and 1 h), and for periods characterized by maximal amounts of UDS in both the normal and AT cultures (2 and 4 h). The results suggest a class of lesions repairable in the normal cells by UDS within 1 h many persist in the AT cells for 4 h or longer.",

author = "Vincent, {R. A.} and Fink, {A. J.} and Huang, {P. C.}",

note = "Funding Information: We thank Dr. S.Y. Wangf or the use of his GammatorM, rs. PatriciaB ohdan for technicaal ssistancea,n d Mrs. Dorothy Smith for typing. This work was supportedb y grant NP-236 from the AmericanC ancerSociety (RAV) and grantR OES1596f romthe N.I.H. (PCH).",

year = "1980",

doi = "10.1016/0027-5107(80)90038-X",

language = "English (US)",

volume = "72",

pages = "245--249",

journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",

issn = "0027-5107",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Unscheduled DNA synthesis in cultured ataxia telangiectasia fibroblast-like cells

AU - Vincent, R. A.

AU - Fink, A. J.

AU - Huang, P. C.

N1 - Funding Information: We thank Dr. S.Y. Wangf or the use of his GammatorM, rs. PatriciaB ohdan for technicaal ssistancea,n d Mrs. Dorothy Smith for typing. This work was supportedb y grant NP-236 from the AmericanC ancerSociety (RAV) and grantR OES1596f romthe N.I.H. (PCH).

PY - 1980

Y1 - 1980

N2 - The capacity of cultured fibroblast-like cells from 5 ataxia telangiectasia (AT) patients to repair γ-ray-induced damage formed in DNA under aerobic conditions was measured by unscheduled DNA synthesis (UDS). The AT cultures exhibited generally reduced UDS compared to the normal cultures for γ-ray doses of 4–12 kR. Reduced UDS in the AT cultures was observed for repair-incubation periods at which UDS was not saturated (0.5 and 1 h), and for periods characterized by maximal amounts of UDS in both the normal and AT cultures (2 and 4 h). The results suggest a class of lesions repairable in the normal cells by UDS within 1 h many persist in the AT cells for 4 h or longer.

AB - The capacity of cultured fibroblast-like cells from 5 ataxia telangiectasia (AT) patients to repair γ-ray-induced damage formed in DNA under aerobic conditions was measured by unscheduled DNA synthesis (UDS). The AT cultures exhibited generally reduced UDS compared to the normal cultures for γ-ray doses of 4–12 kR. Reduced UDS in the AT cultures was observed for repair-incubation periods at which UDS was not saturated (0.5 and 1 h), and for periods characterized by maximal amounts of UDS in both the normal and AT cultures (2 and 4 h). The results suggest a class of lesions repairable in the normal cells by UDS within 1 h many persist in the AT cells for 4 h or longer.

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U2 - 10.1016/0027-5107(80)90038-X

DO - 10.1016/0027-5107(80)90038-X

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SP - 245

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JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

IS - 2

ER -

Unscheduled DNA synthesis in cultured ataxia telangiectasia fibroblast-like cells

Abstract

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