TY - JOUR
T1 - Unreliability of the microcomplement fixation method for Xg(a) typing of cultured fibroblasts
AU - Hsu, S. H.
AU - Migeon, B. R.
AU - Bias, W. B.
PY - 1976/12/1
Y1 - 1976/12/1
N2 - Evidence pointing to localization of the Xg locus on either the long or short arm of the X chromosome is conflicting. Whether the Xg locus is subject to X inactivation is also undecided. A report that Xg antigen could be detected on cultured fibroblasts or in hybridized cell lines by microcomplement fixation seemed to open new possibilities of studying these important problems. Indeed, Fellous et al., using the microcomplement fixation method, mapped the Xg locus to the short arm of the X chromosome by typing a human cell line involving a translocation, t(X;17)(p11;q20), of the X and chromosome 17. However, the authors were unable to duplicate the results of Fellous et al., whether they used their technique or their own.
AB - Evidence pointing to localization of the Xg locus on either the long or short arm of the X chromosome is conflicting. Whether the Xg locus is subject to X inactivation is also undecided. A report that Xg antigen could be detected on cultured fibroblasts or in hybridized cell lines by microcomplement fixation seemed to open new possibilities of studying these important problems. Indeed, Fellous et al., using the microcomplement fixation method, mapped the Xg locus to the short arm of the X chromosome by typing a human cell line involving a translocation, t(X;17)(p11;q20), of the X and chromosome 17. However, the authors were unable to duplicate the results of Fellous et al., whether they used their technique or their own.
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M3 - Article
C2 - 1024643
AN - SCOPUS:0017205882
VL - 16
SP - 382
EP - 386
JO - Cytogenetic and Genome Research
JF - Cytogenetic and Genome Research
SN - 1424-8581
IS - 1-5
ER -