Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment

Li Yin Huang, George Umanah, Melinda Hauser, Cagdas Son, Boris Arshava, Fred Naider, Jeffrey M. Becker

Research output: Contribution to journalArticle

Abstract

Ste2p is the G protein-coupled receptor (GPCR) for the tridecapeptide pheromone α factor of Saccharomyces cerevisiae. This receptor-pheromone pair has been used extensively as a paradigm for investigating GPCR structure and function. Expression in yeast harboring a cognate tRNA/aminoacyl-tRNA synthetase pair specifically evolved to incorporate p-benzoyl-L-phenylalanine (Bpa) in response to the amber codon allowed the biosynthesis of Bpa-substituted Ste2p in its native cell. We replaced natural amino acid residues in Ste2p with Bpa by engineering amber TAG stop codons into STE2 encoded on a plasmid. Several of the expressed Bpa-substituted Ste2p receptors exhibited high-affinity ligand binding, and incorporation of Bpa into Ste2p influenced biological activity as measured by growth arrest of whole cells in response to α factor. We found that, at concentrations of 0.1-0.5 mM, a dipeptide containing Bpa could be used to enhance delivery of Bpa into the cell, while at 2 mM, both dipeptide and Bpa were equally effective. The application of a peptide delivery system for unnatural amino acids will extend the use of the unnatural amino acid replacement methodology to amino acids that are impermeable to yeast. Incorporation of Bpa into Ste2p was verified by mass spectrometric analysis, and two Bpa-Ste2p mutants were able to selectively capture α factor into the ligand-binding site after photoactivation. To our knowledge, this is the first experimental evidence documenting an unnatural amino acid replacement in a GPCR expressed in its native environment and the use of a mutated receptor to photocapture a peptide ligand.

Original languageEnglish (US)
Pages (from-to)5638-5648
Number of pages11
JournalBiochemistry
Volume47
Issue number20
DOIs
StatePublished - May 20 2008
Externally publishedYes

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Fungal Proteins
G-Protein-Coupled Receptors
Phenylalanine
Yeast
Amino Acids
Terminator Codon
Dipeptides
Ligands
Pheromone Receptors
Yeasts
Amber
Amino Acyl-tRNA Synthetases
Peptides
Pheromones
Biosynthesis
Transfer RNA
Bioactivity
Saccharomyces cerevisiae
Plasmids
Binding Sites

ASJC Scopus subject areas

  • Biochemistry

Cite this

Huang, L. Y., Umanah, G., Hauser, M., Son, C., Arshava, B., Naider, F., & Becker, J. M. (2008). Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment. Biochemistry, 47(20), 5638-5648. https://doi.org/10.1021/bi701866e

Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment. / Huang, Li Yin; Umanah, George; Hauser, Melinda; Son, Cagdas; Arshava, Boris; Naider, Fred; Becker, Jeffrey M.

In: Biochemistry, Vol. 47, No. 20, 20.05.2008, p. 5638-5648.

Research output: Contribution to journalArticle

Huang, LY, Umanah, G, Hauser, M, Son, C, Arshava, B, Naider, F & Becker, JM 2008, 'Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment', Biochemistry, vol. 47, no. 20, pp. 5638-5648. https://doi.org/10.1021/bi701866e
Huang, Li Yin ; Umanah, George ; Hauser, Melinda ; Son, Cagdas ; Arshava, Boris ; Naider, Fred ; Becker, Jeffrey M. / Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment. In: Biochemistry. 2008 ; Vol. 47, No. 20. pp. 5638-5648.
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