Treatment of human lymphoid tissue-culture cells and fresh human lymphocytes withVibrio choleraeneuraminidase (NASE) underconditions that removed 65% of the cell-surface sialic acid uncoveredsurface structures capable of reacting with substances, probably antibodies, in normal rabbit serum. Three to five times more of these surface componentswere detectable on NASE-treated cultured lymphoid cells than on untreated cells. The antibody in normal rabbit serum could be removed byabsorption with NASE-treated lymphoid cells in the presence of ethylene-diamine tetraethylacetate without lowering complement levels.—J NatCancer Inst 48: 1271-1276, 1972.
ASJC Scopus subject areas
- Cancer Research