Unique features of human basophilic granulocytes developed in in vitro culture

Teruko Ishizaka, Daniel H. Conrad, Thomas F. Huff, Dean D. Metcalfe, Richard L. Stevens, Robert A. Lewis

Research output: Contribution to journalArticle

Abstract

Human basophilic granulocytes were obtained in suspension culture of mononuclear cells from umbilical cord blood. Precursors of basophils in the cord blood are nonadherent cells and bear neither surface Ig nor T cell marker. Cultured basophils contain chondroitin-4-sulfate proteoglycan, 1.74 μg histamine per 106 cells in average, and bear 120,000-380,000 IgE receptors per cell. The IgE receptor molecule has a molecular weight of approximately 64,000 daltons. Human, rat and mouse IgE bind to the receptors with comparable high affinity and passively sensitize the cells for mediator release. Challenge of sensitized basophils with anti-IgE resulted in stimulation of phospholipid methylation, 45Ca uptake, release of both histamine and free arachidonic acid, and enhancement of phosphatidylinositol (PI) turnover. Evidence was obtained that the activation of membrane-associated proteolytic enzyme and methyltransferases is involved in subsequent IgE-mediated biochemical cascades such as PI turnover, Ca2+ uptake and mediator release.

Original languageEnglish (US)
Pages (from-to)137-143
Number of pages7
JournalInternational Archives of Allergy and Immunology
Volume77
Issue number1-2
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Ishizaka, T., Conrad, D. H., Huff, T. F., Metcalfe, D. D., Stevens, R. L., & Lewis, R. A. (1985). Unique features of human basophilic granulocytes developed in in vitro culture. International Archives of Allergy and Immunology, 77(1-2), 137-143. https://doi.org/10.1159/000233768